TY - JOUR
T1 - Evaluation of a new real-time PCR assay (Check-Direct CPE) for rapid detection of KPC, OXA-48, VIM, and NDM carbapenemases using spiked rectal swabs
AU - Nijhuis, R.
AU - Samuelsen, O.
AU - Savelkoul, P.
AU - van Zwet, A.
PY - 2013/1/1
Y1 - 2013/1/1
N2 - To prevent the spread of carbapenemase-producing bacteria, a fast and accurate detection of patients carrying these bacteria is extremely important. The Check-Direct CPE assay (Check-Points, Wageningen, The Netherlands) is a new multiplex real-time PCR assay, which has been developed to detect and differentiate between the most prevalent carbapenemase genes encountered in Enterobacteriaceae (bla(KPC), bla(OXA-48), bla(VIM), and bla(NDM)) directly from rectal swabs. Evaluation of this assay using 83 non-duplicate isolates demonstrated 100% sensitivity and specificity and the correct identification of the carbapenemase gene(s) present in all carbapenemase-producing isolates. Moreover, the limit of detection (LoD) of the real-time PCR assay in spiked rectal swabs was determined and showed comparable LoDs with the ChromID CARBA agar. With an excellent performance on clinical isolates and spiked rectal swabs, this assay appeared to be an accurate and rapid method to detect bla(KPC), bla(OXA-48), bla(VIM), and bla(NDM) genes directly from a rectal screening swab. (C) 2013 Elsevier Inc. All rights reserved.
AB - To prevent the spread of carbapenemase-producing bacteria, a fast and accurate detection of patients carrying these bacteria is extremely important. The Check-Direct CPE assay (Check-Points, Wageningen, The Netherlands) is a new multiplex real-time PCR assay, which has been developed to detect and differentiate between the most prevalent carbapenemase genes encountered in Enterobacteriaceae (bla(KPC), bla(OXA-48), bla(VIM), and bla(NDM)) directly from rectal swabs. Evaluation of this assay using 83 non-duplicate isolates demonstrated 100% sensitivity and specificity and the correct identification of the carbapenemase gene(s) present in all carbapenemase-producing isolates. Moreover, the limit of detection (LoD) of the real-time PCR assay in spiked rectal swabs was determined and showed comparable LoDs with the ChromID CARBA agar. With an excellent performance on clinical isolates and spiked rectal swabs, this assay appeared to be an accurate and rapid method to detect bla(KPC), bla(OXA-48), bla(VIM), and bla(NDM) genes directly from a rectal screening swab. (C) 2013 Elsevier Inc. All rights reserved.
U2 - 10.1016/j.diagmicrobio.2013.09.007
DO - 10.1016/j.diagmicrobio.2013.09.007
M3 - Article
C2 - 24135412
SN - 0732-8893
VL - 77
SP - 316
EP - 320
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 4
ER -