ESHRE PGD consortium best practice guidelines for fluorescence in situ hybridization-based PGD

G. L. Harton*, Joyce C. Harper, E. Coonen, T. Pehlivan, K. Vesela, Leeanda J. Wilton

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


In 2005, the European Society for Human Reproduction and Embryology (ESHRE) PGD Consortium published a set of Guidelines for Best Practice PGD to give information, support and guidance to potential, existing and fledgling PGD programmes. The subsequent years have seen the introduction of new technologies as well as evolution of current techniques. Additionally, in light of recent advice from ESHRE on how practice guidelines should be written and formulated, the Consortium believed it was timely to revise and update the PGD guidelines. Rather than one document that covers all of PGD, the new guidelines are separated into four new documents that apply to different aspects of a PGD programme, i.e. organization of a PGD centre, fluorescence in situ hybridization (FISH)-based testing, amplification-based testing and polar body and embryo biopsy for PGD/preimplantation genetic screening (PGS). Here, we have updated the sections that pertain to FISH-based PGD. PGS has become a highly controversial technique. Opinions of laboratory specialists and clinicians interested in PGD and PGS have been taken into account here. Whereas some believe that PGS does not have a place in clinical medicine, others disagree; therefore, PGS has been included. This document should assist everyone interested in PGD/PGS in developing the best laboratory and clinical practice possible. Topics covered in this guideline include inclusion/exclusion criteria for FISH-based PGD testing, referrals and genetic counselling, preclinical validation of tests, FISH-based testing methods, spreading of cells for analysis, set-up of local IVF centre and transport PGD centres, quality control/quality assurance and diagnostic confirmation of untransferred embryos.
Original languageEnglish
Pages (from-to)25-32
JournalHuman Reproduction
Issue number1
Publication statusPublished - Jan 2011


  • European Society for Human Reproduction and Embryology
  • PGD
  • fluorescence in situ hybridization
  • preimplantation genetic screening
  • misdiagnosis


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