Epigenomic profiling of DNA methylation in paired prostate cancer versus adjacent benign tissue

Milan S. Geybels, Shanshan Zhao, Chao-Jen Wong, Marina Bibikova, Brandy Klotzle, Michael Wu, Elaine A. Ostrander, Jian-Bing Fan, Ziding Feng, Janet L. Stanford*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

33 Citations (Web of Science)

Abstract

Background Aberrant DNA methylation may promote prostate carcinogenesis. We investigated epigenome-wide DNA methylation profiles in prostate cancer (PCa) compared to adjacent benign tissue to identify differentially methylated CpG sites. MethodsThe study included paired PCa and adjacent benign tissue samples from 20 radical prostatectomy patients. Epigenetic profiling was done using the Infinium HumanMethylation450 BeadChip. Linear models that accounted for the paired study design and False Discovery Rate Q-values were used to evaluate differential CpG methylation. mRNA expression levels of the genes with the most differentially methylated CpG sites were analyzed. ResultsIn total, 2,040 differentially methylated CpG sites were identified in PCa versus adjacent benign tissue (Q-value
Original languageEnglish
Pages (from-to)1941-1950
JournalProstate
Volume75
Issue number16
DOIs
Publication statusPublished - 1 Dec 2015

Keywords

  • prostate cancer
  • DNA methylation
  • mRNA expression
  • tumor
  • benign

Cite this