Enthesis Healing Is Dependent on Scaffold Interphase Morphology-Results from a Rodent Patellar Model

Carlos J Peniche Silva*, Sebastian A Müller, Nicholas Quirk, Patrina S P Poh, Carla Mayer, Antonella Motta, Claudio Migliaresi, Michael J Coenen, Christopher H Evans, Elizabeth R Balmayor, Martijn van Griensven

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The use of multiphasic scaffolds to treat injured tendon-to-bone entheses has shown promising results in vitro. Here, we used two versions of a biphasic silk fibroin scaffold to treat an enthesis defect created in a rat patellar model in vivo. One version presented a mixed transition between the bony and the tendon end of the construct (S-MT) while this transition was abrupt in the second version (S-AT). At 12 weeks after surgery, the S-MT scaffold promoted better healing of the injured enthesis, with minimal undesired ossification of the insertion area. The expression of tenogenic and chondrogenic markers was sustained for longer in the S-MT-treated group and the tangent modulus of the S-MT-treated samples was similar to the native tissue at 12 weeks while that of the S-AT-treated enthesis was lower. Our study highlights the important role of the transition zone of multiphasic scaffolds in the treatment of complex interphase tissues such as the tendon-to-bone enthesis.

Original languageEnglish
Article number1752
Number of pages22
JournalCells
Volume11
Issue number11
DOIs
Publication statusPublished - 26 May 2022

Keywords

  • enthesis
  • scaffold
  • multiphasic
  • silk fibroin
  • tendon
  • ANTERIOR CRUCIATE LIGAMENT
  • SILK FIBROIN SCAFFOLDS
  • IN-VIVO EVALUATION
  • NANOFIBER SCAFFOLD
  • BONE-FORMATION
  • TENDON INJURY
  • REPAIR
  • CELLS
  • EXPRESSION
  • RAT

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