TY - JOUR
T1 - Enhanced Nucleotide Excision Repair in Human Fibroblasts Pre-exposed to Ionizing Radiation
AU - Cramers, Patricia
AU - Filon, Alfred Ronald
AU - Pines, Alex
AU - Kleinjans, Jos C.
AU - Mullenders, Leon H. F.
AU - van Zeeland, Albert A.
PY - 2012
Y1 - 2012
N2 - Cellular protection against deleterious effects of DNA damaging agents requires an intricate network of defense mechanisms known as the DNA damage response (DDR). Ionizing radiation (IR) mediated activation of the DDR induces a transcriptional upregulation of genes that are also involved in nucleotide excision repair (NER). This suggests that pre-exposure to X-rays might stimulate NER in human cells. Here, we demonstrate in normal human fibroblasts that UV-induced NER is augmented by pre-exposure to IR and that this increased repair is accompanied by elevated mRNA and protein levels of the NER factors XPC and DDB2. Furthermore, when IR exposure precedes local UV irradiation, the presence of XPC and DDB2 at the sites of local UV damages is increased. This increase might be p53 dependent, but the mechanism of X-ray specific stabilization of p53 is unclear as both X-rays and UV stabilize p53.
AB - Cellular protection against deleterious effects of DNA damaging agents requires an intricate network of defense mechanisms known as the DNA damage response (DDR). Ionizing radiation (IR) mediated activation of the DDR induces a transcriptional upregulation of genes that are also involved in nucleotide excision repair (NER). This suggests that pre-exposure to X-rays might stimulate NER in human cells. Here, we demonstrate in normal human fibroblasts that UV-induced NER is augmented by pre-exposure to IR and that this increased repair is accompanied by elevated mRNA and protein levels of the NER factors XPC and DDB2. Furthermore, when IR exposure precedes local UV irradiation, the presence of XPC and DDB2 at the sites of local UV damages is increased. This increase might be p53 dependent, but the mechanism of X-ray specific stabilization of p53 is unclear as both X-rays and UV stabilize p53.
U2 - 10.1111/j.1751-1097.2011.01019.x
DO - 10.1111/j.1751-1097.2011.01019.x
M3 - Article
C2 - 22017241
SN - 0031-8655
VL - 88
SP - 147
EP - 153
JO - Photochemistry and Photobiology
JF - Photochemistry and Photobiology
IS - 1
ER -