A material-based bottom-up approach is proposed towards an assembly of cells and engineered micro-objects at the macroscale. We show how shape, size and wettability of engineered micro-objects play an important role in the behavior of cells on these objects. This approach can, among other applications, be used as a tool to engineer complex 3D tissues of clinically relevant size.
- bottom-up tissue engineering
- human mesenchymal stromal cells
- cell aggregates
Leferink, A., Schipper, D., Arts, E., Vrij, E., Rivron, N., Karperien, M., Mittmann, K., van Blitterswijk, C., Moroni, L., & Truckenmueller, R. (2014). Engineered Micro-Objects as Scaffolding Elements in Cellular Building Blocks for Bottom-Up Tissue Engineering Approaches. Advanced Materials, 26(16), 2592-2599. https://doi.org/10.1002/adma.201304539