Endurance Exercise Increases Intestinal Uptake of the Peanut Allergen Ara h 6 after Peanut Consumption in Humans

Lonneke M. Janssen Duijghuijsen, Klaske van Norren, Sander Grefte, Stef J. Koppelman, Kaatje Lenaerts, Jaap Keijer, Renger F. Witkamp, Harry J. Wichers*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Controlled studies on the effect of exercise on intestinal uptake of protein are scarce and underlying mechanisms largely unclear. We studied the uptake of the major allergen Ara h 6 following peanut consumption in an exercise model and compared this with changes in markers of intestinal permeability and integrity. Ten overnight-fasted healthy non-allergic men (n = 4) and women (n = 6) (23 +/- 4 years) ingested 100 g of peanuts together with a lactulose/rhamnose (L/R) solution, followed by rest or by 60 min cycling at 70% of their maximal workload. Significantly higher, though variable, levels of Ara h 6 in serum were found during exercise compared to rest (Peak p = 0.03; area under the curve p = 0.006), with individual fold changes ranging from no increase to an increase of over 150-fold in the uptake of Ara h 6. Similarly, uptake of lactulose (2-18 fold change, p = 0.0009) and L/R ratios (0.4-7.9 fold change, p = 0.04) were significantly increased which indicates an increase in intestinal permeability. Intestinal permeability and uptake of Ara h 6 were strongly correlated (r = 0.77, p <0.0001 for lactulose and Ara h 6). Endurance exercise after consumption may lead to increased paracellular intestinal uptake of food proteins.

Original languageEnglish
Article number84
Number of pages11
JournalNutrients
Volume9
Issue number1
DOIs
Publication statusPublished - Jan 2017

Keywords

  • intestinal permeability
  • Ara h 6
  • allergen
  • endurance exercise
  • food-dependent exercise-induced anaphylaxis
  • DIGESTION-RESISTANT PEPTIDES
  • HUMAN-SERUM
  • PERMEABILITY
  • PROTEIN
  • BLOOD
  • FOOD
  • GUT
  • MEN
  • ABSORPTION
  • STABILITY

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