Emergency (clonal spread) of methicillin-resistant Staphylococcus aureus (MRSA), extended spectrum (ESBL) - and AmpC beta-lactamase-producing Gram-negative bacteria infections at Pediatric Department, Bosnia and Herzegovina

S. Uzunovic, B. Bedenic, A. Budimir, F. Kamberovic, A. Ibrahimagic, S. Delic-Bikic, S. Sivec, T. Mestrovic, D. Varda Brkic, M.I.A. Rijnders, E.E. Stobberingh

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Aim of this study was to investigate the prevalence of methicillin-resistant Staphylococcus aureus (MRSA), extended-spectrum (ESBL) and plasmid-mediated AmpC beta-lactamase producing Gram-negative bacteria in children.

Antibiotic susceptibility of MRSA and beta-lactamase producing Gram-negative bacteria was determined by disc diffusion and broth microdilution methods according to CLSI guidelines. Methicillin resistance was confirmed by the presence of mecA gene by PCR. The genetic characterization of S. aures was performed using spa-typing and the algorithm based upon repeat pattern (BURP). Double-disk synergy test was used to screen for ESBL production. PCR was used to detect (bla) ESBL alleles. Genetic relatedness of the strains was tested by pulsed-field gel electrophoresis (PFGE).

Among 23 MRSA, 12 (52.2 %) were obtained from newborns. MLST CC152 (spa-CC 355-595) (Balkan clone) was the most prevalent, 20 (87 %) cases. Among 24 beta-lactamase producing Gram-negative bacteria, 10 (41.7 %) were obtained from each newborns and one-year-old children; 14 (58.3 %) were from urine. Among 11 Klebsiella strains isolated from urine eight (73 %) produced CTX-M-15, and one CTX-M-3 beta-lactamase. Twenty (83 %) of CTX-M producers were coproduced by other types of beta-lactamases. Fifteen (65.2 %) MRSA isolates were clonally related. Five clones among 13 K. pneumoniae isolates were detected by PFGE suggesting clonal spread of beta-lactamase producing Gram-negative bacteria.

Pediatric infections caused by clonal spread of MRSA and beta-lactamase-producing Gram-negative bacteria are of major concern. Proper infection control measures should be implemented in order to avoid the transmission and major outbreaks.

Original languageEnglish
Pages (from-to)747-756
Number of pages10
JournalWiener Klinische Wochenschrift
Volume126
Issue number23-24
DOIs
Publication statusPublished - Dec 2014

Keywords

  • CTX-M-15
  • Burp
  • Balkan clone
  • beta-lactamase
  • Spa-typing
  • Antibiotic resistance
  • CTX-M-TYPE
  • ESCHERICHIA-COLI
  • MOLECULAR CHARACTERIZATION
  • KLEBSIELLA-PNEUMONIAE
  • POPULATION-DYNAMICS
  • PREVALENCE
  • SPA
  • NETHERLANDS
  • HOSPITALS
  • PATTERNS

Cite this

Uzunovic, S., Bedenic, B., Budimir, A., Kamberovic, F., Ibrahimagic, A., Delic-Bikic, S., Sivec, S., Mestrovic, T., Varda Brkic, D., Rijnders, M. I. A., & Stobberingh, E. E. (2014). Emergency (clonal spread) of methicillin-resistant Staphylococcus aureus (MRSA), extended spectrum (ESBL) - and AmpC beta-lactamase-producing Gram-negative bacteria infections at Pediatric Department, Bosnia and Herzegovina. Wiener Klinische Wochenschrift, 126(23-24), 747-756. https://doi.org/10.1007/s00508-014-0597-2