Effects of supercritical carbon dioxide sterilization on polysaccharidic membranes for surgical applications

F. Scognamiglio*, M. Blanchy, M. Borgogna, A. Travan, I. Donati, J. W. A. M. Bosmans, M. P. Foulc, N. D. Bouvy, S. Paoletti, E. Marsich

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Sterilization methods such as gamma-irradiation, steam sterilization and ethylene oxide gas treatment can have negative effects on molecular structure and properties of polysaccharide-based biomaterials. In this perspective, the use of supercritical carbon dioxide (scCO(2)) has been proposed as an alternative method for biomaterial sterilization. In this work, chemical, mechanical and biological properties of polysaccharidic membranes for surgical applications were investigated after sterilization by scCO(2). Four sets of sterilizing conditions were considered and SEC analyses were performed in order to identify the one with lower impact on the polysaccharidic matrix of membranes (alginate). Mechanical tests showed that the resistance of membranes was slightly affected after sterilization. Biological analyses proved the biocompatibility of the sterilized membranes both in vitro and in a preliminary in vivo test. Overall, this study points out that this sterilization technique can be successfully employed to achieve an effective and safe sterilization of polysaccharidic membranes for surgical use. (C) 2017 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)482-488
Number of pages7
JournalCarbohydrate Polymers
Volume173
DOIs
Publication statusPublished - 1 Oct 2017

Keywords

  • Membranes Alginate Sterilization Hydrogen peroxide (H2O2)
  • Supercritical carbon dioxide (scCO(2))
  • TERMINAL STERILIZATION
  • BACTERIAL-SPORES
  • ETHYLENE-OXIDE
  • MECHANICAL-PROPERTIES
  • SURFACE-PROPERTIES
  • HYDROGEN-PEROXIDE
  • HYALURONIC-ACID
  • MEDICAL DEVICES
  • INACTIVATION
  • CO2

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