Effects of gut microbiota manipulation on ex vivo lipolysis in human abdominal subcutaneous adipocytes

Johan W. E. Jocken; Dorien Reijnders; Emanuel E. Canfora; Mark V. Boekschoten; Joghum Plat; Gijs H. Goossens; Ellen E. Blaak

doi:10.1080/21623945.2018.1464366

Effects of gut microbiota manipulation on ex vivo lipolysis in human abdominal subcutaneous adipocytes

Johan W. E. Jocken^*, Dorien Reijnders, Emanuel E. Canfora, Mark V. Boekschoten, Joghum Plat, Gijs H. Goossens, Ellen E. Blaak

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The intestinal microbiota may contribute to the development of obesity by affecting host lipid metabolism and insulin sensitivity. To investigate the effects of microbiota manipulation on ex vivo basal and beta-adrenergically-stimulated lipolysis in human adipocytes, 36 obese men were randomized to amoxicillin (broad-spectrum antibiotic), vancomycin (narrow-spectrum antibiotic) or placebo treatment (7 d, 1500 mg/d). Before and after treatment, ex vivo adipose tissue lipolysis was assessed under basal conditions and during stimulation with the non-selective beta-agonist isoprenaline using freshly isolated mature adipocytes. Gene (targeted microarray) and protein expression were analyzed to investigate underlying pathways. Antibiotics treatment did not significantly affect basal and maximal isoprenaline-mediated glycerol release from adipocytes. Adipose tissue beta-adrenoceptor expression or post-receptor signalling was also not different between groups. In conclusion, 7 d oral antibiotics treatment has no effect on ex vivo lipolysis in mature adipocytes derived from adipose tissue of obese insulin resistant men.

Original language	English
Pages (from-to)	106-112
Number of pages	7
Journal	Adipocyte
Volume	7
Issue number	2
DOIs	https://doi.org/10.1080/21623945.2018.1464366
Publication status	Published - 1 Jan 2018

Keywords

Microbiota
Lipolysis
Fatty acid metabolism
Adipose Tissue
Obesity
Insulin resistance
HORMONE-SENSITIVE LIPASE
ADIPOSE TRIGLYCERIDE LIPASE
DIET-INDUCED OBESITY
FREE FATTY-ACIDS
PROPIONIC-ACID
ACETATE
PROTEIN
PHOSPHORYLATION
DIFFERENTIATION
EXPRESSION

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10.1080/21623945.2018.1464366

Cite this

@article{273ea57f1a564bc1a1a46f72331c0ac4,

title = "Effects of gut microbiota manipulation on ex vivo lipolysis in human abdominal subcutaneous adipocytes",

abstract = "The intestinal microbiota may contribute to the development of obesity by affecting host lipid metabolism and insulin sensitivity. To investigate the effects of microbiota manipulation on ex vivo basal and beta-adrenergically-stimulated lipolysis in human adipocytes, 36 obese men were randomized to amoxicillin (broad-spectrum antibiotic), vancomycin (narrow-spectrum antibiotic) or placebo treatment (7 d, 1500 mg/d). Before and after treatment, ex vivo adipose tissue lipolysis was assessed under basal conditions and during stimulation with the non-selective beta-agonist isoprenaline using freshly isolated mature adipocytes. Gene (targeted microarray) and protein expression were analyzed to investigate underlying pathways. Antibiotics treatment did not significantly affect basal and maximal isoprenaline-mediated glycerol release from adipocytes. Adipose tissue beta-adrenoceptor expression or post-receptor signalling was also not different between groups. In conclusion, 7 d oral antibiotics treatment has no effect on ex vivo lipolysis in mature adipocytes derived from adipose tissue of obese insulin resistant men.",

keywords = "Microbiota, Lipolysis, Fatty acid metabolism, Adipose Tissue, Obesity, Insulin resistance, HORMONE-SENSITIVE LIPASE, ADIPOSE TRIGLYCERIDE LIPASE, DIET-INDUCED OBESITY, FREE FATTY-ACIDS, PROPIONIC-ACID, ACETATE, PROTEIN, PHOSPHORYLATION, DIFFERENTIATION, EXPRESSION",

author = "Jocken, {Johan W. E.} and Dorien Reijnders and Canfora, {Emanuel E.} and Boekschoten, {Mark V.} and Joghum Plat and Goossens, {Gijs H.} and Blaak, {Ellen E.}",

year = "2018",

month = jan,

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doi = "10.1080/21623945.2018.1464366",

language = "English",

volume = "7",

pages = "106--112",

journal = "Adipocyte",

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T1 - Effects of gut microbiota manipulation on ex vivo lipolysis in human abdominal subcutaneous adipocytes

AU - Jocken, Johan W. E.

AU - Reijnders, Dorien

AU - Canfora, Emanuel E.

AU - Boekschoten, Mark V.

AU - Plat, Joghum

AU - Goossens, Gijs H.

AU - Blaak, Ellen E.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - The intestinal microbiota may contribute to the development of obesity by affecting host lipid metabolism and insulin sensitivity. To investigate the effects of microbiota manipulation on ex vivo basal and beta-adrenergically-stimulated lipolysis in human adipocytes, 36 obese men were randomized to amoxicillin (broad-spectrum antibiotic), vancomycin (narrow-spectrum antibiotic) or placebo treatment (7 d, 1500 mg/d). Before and after treatment, ex vivo adipose tissue lipolysis was assessed under basal conditions and during stimulation with the non-selective beta-agonist isoprenaline using freshly isolated mature adipocytes. Gene (targeted microarray) and protein expression were analyzed to investigate underlying pathways. Antibiotics treatment did not significantly affect basal and maximal isoprenaline-mediated glycerol release from adipocytes. Adipose tissue beta-adrenoceptor expression or post-receptor signalling was also not different between groups. In conclusion, 7 d oral antibiotics treatment has no effect on ex vivo lipolysis in mature adipocytes derived from adipose tissue of obese insulin resistant men.

AB - The intestinal microbiota may contribute to the development of obesity by affecting host lipid metabolism and insulin sensitivity. To investigate the effects of microbiota manipulation on ex vivo basal and beta-adrenergically-stimulated lipolysis in human adipocytes, 36 obese men were randomized to amoxicillin (broad-spectrum antibiotic), vancomycin (narrow-spectrum antibiotic) or placebo treatment (7 d, 1500 mg/d). Before and after treatment, ex vivo adipose tissue lipolysis was assessed under basal conditions and during stimulation with the non-selective beta-agonist isoprenaline using freshly isolated mature adipocytes. Gene (targeted microarray) and protein expression were analyzed to investigate underlying pathways. Antibiotics treatment did not significantly affect basal and maximal isoprenaline-mediated glycerol release from adipocytes. Adipose tissue beta-adrenoceptor expression or post-receptor signalling was also not different between groups. In conclusion, 7 d oral antibiotics treatment has no effect on ex vivo lipolysis in mature adipocytes derived from adipose tissue of obese insulin resistant men.

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KW - Fatty acid metabolism

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KW - Obesity

KW - Insulin resistance

KW - HORMONE-SENSITIVE LIPASE

KW - ADIPOSE TRIGLYCERIDE LIPASE

KW - DIET-INDUCED OBESITY

KW - FREE FATTY-ACIDS

KW - PROPIONIC-ACID

KW - ACETATE

KW - PROTEIN

KW - PHOSPHORYLATION

KW - DIFFERENTIATION

KW - EXPRESSION

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DO - 10.1080/21623945.2018.1464366

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