TY - JOUR
T1 - Effects of Cigarette Smoke on Adipose and Skeletal Muscle Tissue
T2 - In Vivo and In Vitro Studies
AU - Wang, Lei
AU - van Iersel, Lieke E J
AU - Pelgrim, Charlotte E
AU - Lu, Jingyi
AU - van Ark, Ingrid
AU - Leusink-Muis, Thea
AU - Gosker, Harry R
AU - Langen, Ramon C J
AU - Schols, Annemie M W J
AU - Argilés, Josep M
AU - van Helvoort, Ardy
AU - Kraneveld, Aletta D
AU - Garssen, Johan
AU - Henricks, Paul A J
AU - Folkerts, Gert
AU - Braber, Saskia
N1 - Funding Information:
The research grant funding was received from the Chinese Scholarship Council for LW, Award NO. 201706170055. This work has been supported by the LSH-TKI-Lung Foundation Netherlands PPP allowance 10.2.16.119.
Publisher Copyright:
© 2022 by the authors.
PY - 2022/9/16
Y1 - 2022/9/16
N2 - Chronic obstructive pulmonary disease (COPD), often caused by smoking, is a chronic lung disease with systemic manifestations including metabolic comorbidities. This study investigates adaptive and pathological alterations in adipose and skeletal muscle tissue following cigarette smoke exposure using in vivo and in vitro models. Mice were exposed to cigarette smoke or air for 72 days and the pre-adipose cell line 3T3-L1 was utilized as an in vitro model. Cigarette smoke exposure decreased body weight, and the proportional loss in fat mass was more pronounced than the lean mass loss. Cigarette smoke exposure reduced adipocyte size and increased adipocyte numbers. Adipose macrophage numbers and associated cytokine levels, including interleukin-1β, interleukine-6 and tumor necrosis factor-α were elevated in smoke-exposed mice. Muscle strength and protein synthesis signaling were decreased after smoke exposure; however, muscle mass was not changed. In vitro studies demonstrated that lipolysis and fatty acid oxidation were upregulated in cigarette smoke-exposed pre-adipocytes. In conclusion, cigarette smoke exposure induces a loss of whole-body fat mass and adipose atrophy, which is likely due to enhanced lipolysis.
AB - Chronic obstructive pulmonary disease (COPD), often caused by smoking, is a chronic lung disease with systemic manifestations including metabolic comorbidities. This study investigates adaptive and pathological alterations in adipose and skeletal muscle tissue following cigarette smoke exposure using in vivo and in vitro models. Mice were exposed to cigarette smoke or air for 72 days and the pre-adipose cell line 3T3-L1 was utilized as an in vitro model. Cigarette smoke exposure decreased body weight, and the proportional loss in fat mass was more pronounced than the lean mass loss. Cigarette smoke exposure reduced adipocyte size and increased adipocyte numbers. Adipose macrophage numbers and associated cytokine levels, including interleukin-1β, interleukine-6 and tumor necrosis factor-α were elevated in smoke-exposed mice. Muscle strength and protein synthesis signaling were decreased after smoke exposure; however, muscle mass was not changed. In vitro studies demonstrated that lipolysis and fatty acid oxidation were upregulated in cigarette smoke-exposed pre-adipocytes. In conclusion, cigarette smoke exposure induces a loss of whole-body fat mass and adipose atrophy, which is likely due to enhanced lipolysis.
KW - Adipose Tissue/metabolism
KW - Animals
KW - Cigarette Smoking
KW - Cytokines/metabolism
KW - Fatty Acids/metabolism
KW - Interleukin-1beta/metabolism
KW - Mice
KW - Muscle, Skeletal/metabolism
KW - Smoke/adverse effects
KW - Tumor Necrosis Factor-alpha/metabolism
U2 - 10.3390/cells11182893
DO - 10.3390/cells11182893
M3 - Article
C2 - 36139468
SN - 2073-4409
VL - 11
JO - Cells
JF - Cells
IS - 18
M1 - 2893
ER -