TY - JOUR
T1 - Effects of a single aerobic exercise on perfused boundary region and microvascular perfusion: a field study
AU - Fuchs, A.
AU - Neumann, T.
AU - Drinhaus, H.
AU - Herrmann, A.
AU - Vink, H.
AU - Annecke, T.
N1 - Funding Information:
Open Access funding enabled and organized by Projekt DEAL. This work was supported by intramural funding of the research group ‘Klinische Forschung und Lehre’ to T.A., University Hospital of Cologne, Department of Anaesthesiology and Intensive Care Medicine, Cologne, Germany.
Publisher Copyright:
© 2021, The Author(s).
PY - 2022/4
Y1 - 2022/4
N2 - The endothelium and the glycocalyx play a pivotal role in regulating microvascular function and perfusion in health and critical illness. It is unknown today, whether aerobic exercise immediately affects dimensions of the endothelial surface layer (ESL) in relation to microvascular perfusion as a physiologic adaption to increased nutritional demands. This monocentric observational study was designed to determine real-time ESL and perfusion measurements of the sublingual microcirculation using sidestream dark field imaging performed in 14 healthy subjects before and after completing a 10 km trial running distance. A novel image acquisition and analysis software automatically analysed the perfused boundary region (PBR), an inverse parameter for red blood cell (RBC) penetration of the ESL, in vessels between 5 and 25 mu m diameter. Microvascular perfusion was assessed by calculating RBC filling percentage. There was no significant immediate effect of exercise on PBR and RBC filling percentage. Linear regression analysis revealed a distinct association between change of PBR and change of RBC filling percentage (regression coefficient beta: - 0.026; 95% confidence interval - 0.043 to - 0.009; p = 0.006). A single aerobic exercise did not induce a change of PBR or RBC filling percentage. The endothelium of the microvasculature facilitates efficient perfusion in vessels reacting with an increased endothelial surface layer.
AB - The endothelium and the glycocalyx play a pivotal role in regulating microvascular function and perfusion in health and critical illness. It is unknown today, whether aerobic exercise immediately affects dimensions of the endothelial surface layer (ESL) in relation to microvascular perfusion as a physiologic adaption to increased nutritional demands. This monocentric observational study was designed to determine real-time ESL and perfusion measurements of the sublingual microcirculation using sidestream dark field imaging performed in 14 healthy subjects before and after completing a 10 km trial running distance. A novel image acquisition and analysis software automatically analysed the perfused boundary region (PBR), an inverse parameter for red blood cell (RBC) penetration of the ESL, in vessels between 5 and 25 mu m diameter. Microvascular perfusion was assessed by calculating RBC filling percentage. There was no significant immediate effect of exercise on PBR and RBC filling percentage. Linear regression analysis revealed a distinct association between change of PBR and change of RBC filling percentage (regression coefficient beta: - 0.026; 95% confidence interval - 0.043 to - 0.009; p = 0.006). A single aerobic exercise did not induce a change of PBR or RBC filling percentage. The endothelium of the microvasculature facilitates efficient perfusion in vessels reacting with an increased endothelial surface layer.
KW - Capillary density
KW - Endothelial surface layer
KW - Glycocalyx
KW - Intravital microscopy
KW - Perfused boundary region
KW - Sidestream darkfield imaging
KW - capillary density
KW - endothelial surface layer
KW - glycocalyx
KW - intravital microscopy
KW - perfused boundary region
KW - sidestream darkfield imaging
KW - YOUNG
KW - ENDOTHELIAL GLYCOCALYX DEGRADATION
KW - INTEGRITY
U2 - 10.1007/s10877-021-00660-w
DO - 10.1007/s10877-021-00660-w
M3 - Article
C2 - 33534119
SN - 1387-1307
VL - 36
SP - 371
EP - 377
JO - Journal of Clinical Monitoring and Computing
JF - Journal of Clinical Monitoring and Computing
IS - 2
ER -