Effective Transfection and Gene Silencing of Primary Murine Macrophages with Small Interfering RNA

Kim van Kuijk, Jan Nagenborg, Judith C Sluimer

Research output: Chapter in Book/Report/Conference proceedingChapterAcademic

Abstract

Transfection of murine primary macrophages to silence genes can be a challenging procedure because this cell type has developed mechanisms to evade cellular intrusion. The introduction of small interfering RNA (siRNA) encapsulated in liposomes to the cell to decrease gene expression is one of the methods that can be used to achieve gene silencing. There are different commercially available compounds to introduce siRNA into the cell, including Lipofectamine RNAiMAX and HiPerfect. The chapter will describe a method for gene silencing in mouse primary macrophages using liposome-based transfection of siRNA.

Original languageEnglish
Title of host publicationAtherosclerosis
Subtitle of host publicationMethods and Protocols
EditorsDipak Ramji
PublisherHumana New York
Pages125-132
Number of pages8
Edition1
ISBN (Electronic)978-1-0716-1924-7
ISBN (Print)978-1-0716-1923-0
DOIs
Publication statusPublished - 2022

Publication series

SeriesMethods in Molecular Biology
ISSN1064-3745

Keywords

  • Animals
  • Gene Silencing
  • Macrophages/metabolism
  • Mice
  • RNA Interference
  • RNA, Small Interfering/genetics
  • Transfection

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