Effect of Trichostatin A on miRNA expression in cultures of primary rat hepatocytes

J. Bolleyn*, J. Fraczek, M. Vinken, D. Lizarraga, S. Gaj, J.H. van Delft, V. Rogiers, T. Vanhaecke

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

In the present study, the effect of Trichostatin A (TSA), a histone deacetylase inhibitor, was investigated on the microRNA (miR, miRNA) expression profile in cultured primary rat hepatocytes by means of microarray analysis. Simultaneously, albumin secretory capacity and morphological features of the hepatocytes were evaluated throughout the culture time. In total, 25 out of 348 miRNAs were found to be differentially expressed between freshly isolated hepatocytes and 7-day cultured cells. Nineteen of these miRNAs were connected with 'general metabolism'. miR-21 and miR-126 were shown to be the most up and down regulated miRs upon cultivation and could be linked to the proliferative response triggered in the hepatocytes upon their isolation from the liver. miR-379 and miR-143, on the other hand, were found to be the most up and down regulated miRs upon TSA treatment. Together with the higher expression of miR-122 observed in TSA-treated versus non-treated cultures, we hypothesize that the changes observed for miR-122, miR-143 and miR-379 could be related to the inhibitory effects of TSA on hepatocellular proliferation.
Original languageEnglish
Pages (from-to)1173-1182
Number of pages10
JournalToxicology in Vitro
Volume25
Issue number6
DOIs
Publication statusPublished - Sep 2011

Keywords

  • MicroRNA
  • Hepatocytes
  • Trichostatin A
  • Histone deacetylase inhibitor
  • Epigenetics
  • LIVER-REGENERATION
  • CYCLIN G1
  • IN-VITRO
  • PCR DATA
  • MICRORNA
  • CANCER
  • GROWTH
  • CELLS
  • DIFFERENTIATION
  • IDENTIFICATION

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