Effect of soluble and insoluble fibers within the in vitro fermentation of chicory root pulp by human gut bacteria

U.S. Ramasamy, K. Venema, H.A. Schols, H. Gruppen

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The aim of this research was to study the in vitro fermentation of chicory root pulp (CRP) and ensiled CRP (ECRP) using human fecal inoculum. Analysis of carbohydrate levels in fermentation digests showed that 51% of all CRP carbohydrates were utilized after 24 h of fermentation. For ECRP, having the same cell wall polysaccharide composition as CRP, but with solubilization of 4 times more of CRP pectin due to ensiling, the fermentation was quicker than with CRP as 11% more carbohydrates were utilized within the first 12 h. The level of fiber utilization for ECRP after 24 h was increased by 8% compared to CRP. This effect on fiber utilization from ECRP seemed to arise from (i) increased levels of soluble pectin fibers (arabinan, homogalacturonan, and galactan) and (ii) ahypothesized more open structure of the remaining cell walls in ECRP, which was more accessible to degradation than the CRP cell wall network.
Original languageEnglish
Pages (from-to)6794-6802
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume62
Issue number28
DOIs
Publication statusPublished - 16 Jul 2014

Keywords

  • chicory root pulp
  • ensued chicory root pulp
  • insoluble fiber
  • soluble fiber
  • fermentation
  • pectin
  • cell wall
  • HUMAN COLONIC MICROBIOTA
  • LARGE-INTESTINE
  • DIETARY MODULATION
  • GENOMIC ANALYSIS
  • SYSTEM
  • OLIGOSACCHARIDES
  • FERMENTABILITY
  • METABOLISM
  • PREBIOTICS
  • CELLULOSE

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