Effect of in vitro culture of human embryos on birthweight of newborns

John C. Dumoulin*, Jolande A. Land, Aafke P. Van Montfoort, Ewka C M Nelissen, Edith Coonen, Josien G. Derhaag, Inge L. Schreurs, Gerard A. Dunselman, Arnold D. Kester, Joep P. Geraedts, Johannes L. Evers

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

249 Citations (Web of Science)


In animal models, in vitro culture of preimplantation embryos has been shown to be a risk factor for abnormal fetal outcome, including high and low birthweight. In the human, mean birthweight of singletons after in vitro fertilization (IVF) is considerably lower than after natural conception, but it is not known whether culture conditions play a role in this. We compared pregnancy rates and perinatal outcomes from singleton pregnancies resulting from a total of 826 first IVF treatment cycles in which oocytes and embryos were randomly allocated to culture in either of two commercially available sequential media systems. When the 110 live born singletons in the Vitrolife group were compared with the 78 singletons in the Cook group, birthweight +/- SEM (3453 +/- 53 versus 3208 +/- 61 g, P = 0.003), and birthweight adjusted for gestational age and gender (mean z-score +/- SEM: 0.13 +/- 0.09 versus -0.31 +/- 0.10, P = 0.001) were both significantly higher in the Vitrolife group. When analyzed by multiple linear regression together with several other variables that could possibly affect birthweight as covariates, the type of culture medium was significantly (P = 0.01) associated with birthweight. In vitro culture of human embryos can affect birthweight of live born singletons.
Original languageEnglish
Pages (from-to)605-612
JournalHuman Reproduction
Issue number3
Publication statusPublished - Mar 2010


  • IVF
  • human
  • perinatal outcome
  • embryo culture
  • birthweight

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