Dynamics of Early Afterdepolarization Mediated Triggered Activity in Cardiac Monolayers

M.G. Chang, C.Y. Chang, E. de Lange, L. Xu, B. O`Rourke, H.S. Karagueuzian, L. Tung, E. Marban, J. N. Weiss, Z. Qu*, M.R. Abraham

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    Abstract

    Early afterdepolarizations (EADs) are voltage oscillations that occur during the repolarizing phase of the cardiac action potential and cause cardiac arrhythmias in a variety of clinical settings. EADs occur in the setting of reduced repolarization reserve and increased inward-over-outward currents, which intuitively explains the repolarization delay but does not mechanistically explain the time-dependent voltage oscillations that are characteristic of EADs. In a recent theoretical study, we identified a dual Hopf-homoclinic bifurcation as a dynamical mechanism that causes voltage oscillations during EADs, depending on the amplitude and kinetics of the L-type Ca2+ channel (LTCC) current relative to the repolarizing K+ currents. Here we demonstrate this mechanism experimentally. We show that cardiac monolayers exposed to the LTCC agonists BayK8644 and isoproterenol produce EAD bursts that are suppressed by the LTCO blocker nitrendipine but not by the Na+ current blocker tetrodoxin, depletion of intracellular Ca2+ stores with thapsigargin and caffeine, or buffering of intracellular Ca2+ with BAPTA-AM. These EAD bursts exhibited a key dynamical signature of the dual Hopf-homoclinic bifurcation mechanism, namely, a gradual slowing in the frequency of oscillations before burst termination. A detailed cardiac action potential model reproduced the experimental observations, and identified intracellular Na+ accumulation as the likely mechanism for terminating EAD bursts. Our findings in cardiac monolayers provide direct support for the Hopf-homoclinic bifurcation mechanism of EAD-mediated triggered activity, and raise the possibility that this mechanism may also contribute to EAD formation in clinical settings such as long QT syndromes, heart failure, and increased sympathetic output.
    Original languageEnglish
    Pages (from-to)1-9
    Number of pages9
    JournalBiophysical Journal
    Volume102
    DOIs
    Publication statusPublished - 2012

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