Dynamic deuterium metabolic imaging in glioblastoma at 7T

Narjes Ahmadian; Mark Gosselink; Sigrid Otto; Dimitri Welting; Kiki Tesselaar; Tom Snijders; Pieter van Eijsden; Jeanine Prompers; Dennis Klomp; Evita Wiegers

doi:10.1007/s10334-025-01299-3

Dynamic deuterium metabolic imaging in glioblastoma at 7T

Narjes Ahmadian^*
, Mark Gosselink
, Sigrid Otto
, Dimitri Welting
, Kiki Tesselaar
, Tom Snijders
, Pieter van Eijsden
, Jeanine Prompers
, Dennis Klomp
, Evita Wiegers

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Objective: This study investigates the dynamic metabolic characteristics of glioblastoma (GBM) using Deuterium Metabolic Imaging (DMI) at 7 T, aiming to dynamically characterize the Warburg effect in vivo. Material and methods: Five newly diagnosed GBM patients underwent dynamic DMI prior to any treatment. 3D ²H free-induction-decay (FID)-Magnetic resonance spectroscopy imaging (MRSI) measurements (11:44 min per scan) were performed at 7 T during ~ 100 min following [6,6’- ²H ₂]glucose consumption. Venous plasma glucose and plasma ²H-Glc atom percent enrichment (APE) levels were measured during the scan. Brain ²H-glucose ( ²H-Glc), ²H-Glutamate/Glutamine ( ²H-Glx), ²H-Lactate ( ²H-Lac), ²H-Lac/ ²H-Glx were analyzed with a two-level (time and tissue type) Linear Mixed Model. Results: Brain ²H-Glc levels were similar across tissue types. ²H-Glx was significantly lower in tumors compared to normal appearing brain tissue (NABT) (p < 0.01). ²H-Lac was significantly higher in tumors compared to NABT (P < 0.01). The ²H-Lac/ ²H-Glx ratio provided tumor-specific contrast, starting 40-50 min post [6,6’- ²H ₂]glucose consumption. Venous plasma glucose and ²H-Glc APE increased within 50 min and venous ²H-Glc APE stabilized at ~ 60%. Discussion: Dynamic DMI at 7 T reveals metabolic alterations in GBM, particularly through the ²H-Lac/ ²H-Glx ratio. This contrast was primarily driven by decreased ²H-Glx rather than profoundly increased ²H-Lac. These findings support the utility of DMI in assessing metabolic reprogramming in brain tumors.

Original language	English
Number of pages	11
Journal	Magnetic Resonance Materials in Physics Biology and Medicine
DOIs	https://doi.org/10.1007/s10334-025-01299-3
Publication status	E-pub ahead of print - 2025

Keywords

Glioblastoma
Deuterium Metabolic Imaging
Glutamate/Glutamine (Glx)
Lactate
BRAIN-TUMORS
TURNOVER
RATES
PET

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@article{dd52f34669494069a4be32e3a6267afa,

title = "Dynamic deuterium metabolic imaging in glioblastoma at 7T",

abstract = "Objective: This study investigates the dynamic metabolic characteristics of glioblastoma (GBM) using Deuterium Metabolic Imaging (DMI) at 7 T, aiming to dynamically characterize the Warburg effect in vivo. Material and methods: Five newly diagnosed GBM patients underwent dynamic DMI prior to any treatment. 3D 2H free-induction-decay (FID)-Magnetic resonance spectroscopy imaging (MRSI) measurements (11:44 min per scan) were performed at 7 T during \textasciitilde{} 100 min following [6,6{\textquoteright}- 2H 2]glucose consumption. Venous plasma glucose and plasma 2H-Glc atom percent enrichment (APE) levels were measured during the scan. Brain 2H-glucose ( 2H-Glc), 2H-Glutamate/Glutamine ( 2H-Glx), 2H-Lactate ( 2H-Lac), 2H-Lac/ 2H-Glx were analyzed with a two-level (time and tissue type) Linear Mixed Model. Results: Brain 2H-Glc levels were similar across tissue types. 2H-Glx was significantly lower in tumors compared to normal appearing brain tissue (NABT) (p < 0.01). 2H-Lac was significantly higher in tumors compared to NABT (P < 0.01). The 2H-Lac/ 2H-Glx ratio provided tumor-specific contrast, starting 40-50 min post [6,6{\textquoteright}- 2H 2]glucose consumption. Venous plasma glucose and 2H-Glc APE increased within 50 min and venous 2H-Glc APE stabilized at \textasciitilde{} 60\%. Discussion: Dynamic DMI at 7 T reveals metabolic alterations in GBM, particularly through the 2H-Lac/ 2H-Glx ratio. This contrast was primarily driven by decreased 2H-Glx rather than profoundly increased 2H-Lac. These findings support the utility of DMI in assessing metabolic reprogramming in brain tumors.",

keywords = "Glioblastoma, Deuterium Metabolic Imaging, Glutamate/Glutamine (Glx), Lactate, BRAIN-TUMORS, TURNOVER, RATES, PET",

author = "Narjes Ahmadian and Mark Gosselink and Sigrid Otto and Dimitri Welting and Kiki Tesselaar and Tom Snijders and \{van Eijsden\}, Pieter and Jeanine Prompers and Dennis Klomp and Evita Wiegers",

year = "2025",

doi = "10.1007/s10334-025-01299-3",

language = "English",

journal = "Magnetic Resonance Materials in Physics Biology and Medicine",

issn = "0968-5243",

publisher = "Springer",

}

TY - JOUR

T1 - Dynamic deuterium metabolic imaging in glioblastoma at 7T

AU - Ahmadian, Narjes

AU - Gosselink, Mark

AU - Otto, Sigrid

AU - Welting, Dimitri

AU - Tesselaar, Kiki

AU - Snijders, Tom

AU - van Eijsden, Pieter

AU - Prompers, Jeanine

AU - Klomp, Dennis

AU - Wiegers, Evita

PY - 2025

Y1 - 2025

N2 - Objective: This study investigates the dynamic metabolic characteristics of glioblastoma (GBM) using Deuterium Metabolic Imaging (DMI) at 7 T, aiming to dynamically characterize the Warburg effect in vivo. Material and methods: Five newly diagnosed GBM patients underwent dynamic DMI prior to any treatment. 3D 2H free-induction-decay (FID)-Magnetic resonance spectroscopy imaging (MRSI) measurements (11:44 min per scan) were performed at 7 T during ~ 100 min following [6,6’- 2H 2]glucose consumption. Venous plasma glucose and plasma 2H-Glc atom percent enrichment (APE) levels were measured during the scan. Brain 2H-glucose ( 2H-Glc), 2H-Glutamate/Glutamine ( 2H-Glx), 2H-Lactate ( 2H-Lac), 2H-Lac/ 2H-Glx were analyzed with a two-level (time and tissue type) Linear Mixed Model. Results: Brain 2H-Glc levels were similar across tissue types. 2H-Glx was significantly lower in tumors compared to normal appearing brain tissue (NABT) (p < 0.01). 2H-Lac was significantly higher in tumors compared to NABT (P < 0.01). The 2H-Lac/ 2H-Glx ratio provided tumor-specific contrast, starting 40-50 min post [6,6’- 2H 2]glucose consumption. Venous plasma glucose and 2H-Glc APE increased within 50 min and venous 2H-Glc APE stabilized at ~ 60%. Discussion: Dynamic DMI at 7 T reveals metabolic alterations in GBM, particularly through the 2H-Lac/ 2H-Glx ratio. This contrast was primarily driven by decreased 2H-Glx rather than profoundly increased 2H-Lac. These findings support the utility of DMI in assessing metabolic reprogramming in brain tumors.

AB - Objective: This study investigates the dynamic metabolic characteristics of glioblastoma (GBM) using Deuterium Metabolic Imaging (DMI) at 7 T, aiming to dynamically characterize the Warburg effect in vivo. Material and methods: Five newly diagnosed GBM patients underwent dynamic DMI prior to any treatment. 3D 2H free-induction-decay (FID)-Magnetic resonance spectroscopy imaging (MRSI) measurements (11:44 min per scan) were performed at 7 T during ~ 100 min following [6,6’- 2H 2]glucose consumption. Venous plasma glucose and plasma 2H-Glc atom percent enrichment (APE) levels were measured during the scan. Brain 2H-glucose ( 2H-Glc), 2H-Glutamate/Glutamine ( 2H-Glx), 2H-Lactate ( 2H-Lac), 2H-Lac/ 2H-Glx were analyzed with a two-level (time and tissue type) Linear Mixed Model. Results: Brain 2H-Glc levels were similar across tissue types. 2H-Glx was significantly lower in tumors compared to normal appearing brain tissue (NABT) (p < 0.01). 2H-Lac was significantly higher in tumors compared to NABT (P < 0.01). The 2H-Lac/ 2H-Glx ratio provided tumor-specific contrast, starting 40-50 min post [6,6’- 2H 2]glucose consumption. Venous plasma glucose and 2H-Glc APE increased within 50 min and venous 2H-Glc APE stabilized at ~ 60%. Discussion: Dynamic DMI at 7 T reveals metabolic alterations in GBM, particularly through the 2H-Lac/ 2H-Glx ratio. This contrast was primarily driven by decreased 2H-Glx rather than profoundly increased 2H-Lac. These findings support the utility of DMI in assessing metabolic reprogramming in brain tumors.

KW - Glioblastoma

KW - Deuterium Metabolic Imaging

KW - Glutamate/Glutamine (Glx)

KW - Lactate

KW - BRAIN-TUMORS

KW - TURNOVER

KW - RATES

KW - PET

U2 - 10.1007/s10334-025-01299-3

DO - 10.1007/s10334-025-01299-3

M3 - Article

SN - 0968-5243

JO - Magnetic Resonance Materials in Physics Biology and Medicine

JF - Magnetic Resonance Materials in Physics Biology and Medicine

ER -

Dynamic deuterium metabolic imaging in glioblastoma at 7T

Abstract

Keywords

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