Does fibrin(ogen) bind to monomeric or dimeric GPVI, or not at all?

Alexandre Slater*, Gina Perrella, Marie-Blanche Onselaer, Eleyna M. Martin, Julia S. Gauer, Rui-Gang Xu, Johan W. M. Heemskerk, Robert A. S. Ariens, Steve P. Watson

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

28 Citations (Web of Science)

Abstract

GPVI is the major signalling receptor for collagen on platelets. Dimerization of GPVI is required for collagen binding and initiation of signalling through the associated FcR-gamma chain. Recently, fibrin and fibrinogen have been identified as ligands for GPVI and shown to induce signalling in support of thrombus formation and stabilization. Contrasting observations have been reported on whether fibrin binds to monomeric or dimeric GPVI, or to neither form. In this article, we discuss reasons for the contradictory results and how to reconcile these. We conclude that a lack of structural knowledge regarding the GPVI constructs that are being used, along with the use of non-standardized reagents, might be the main cause of the discrepant results. This article aims to highlight some of the key areas that need to be addressed.

Original languageEnglish
Pages (from-to)281-289
Number of pages9
JournalPlatelets
Volume30
Issue number3
DOIs
Publication statusPublished - 3 Apr 2019

Keywords

  • Collagen
  • fibrin(ogen)
  • GPVI
  • platelets
  • PLATELET GLYCOPROTEIN VI
  • RECEPTOR-GAMMA-CHAIN
  • SRC FAMILY KINASES
  • C-TYPE LECTIN
  • COLLAGEN RECEPTOR
  • SYSTEMS-APPROACH
  • TYROSINE PHOSPHORYLATION
  • THROMBUS FORMATION
  • CRYSTAL-STRUCTURE
  • ACTIVATION

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