DNA-SMART: Biopatterned Polymer Film Microchannels for Selective Immobilization of Proteins and Cells

Ann-Kathrin Schneider, Pavel M. Nikolov, Stefan Giselbrecht, Christof M. Niemeyer*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

A novel SMART module, dubbed "DNA-SMART" (DNA substrate modification and replication by thermoforming) is reported, where polymer films are premodified with single-stranded DNA capture strands, microthermo formed into 3L) structures, and postmodified with complementary DNA-protein conjugates to realize complex biologically active surfaces within microfluidic devices. As a proof of feasibility, it is demonstrated that microchannels presenting three different proteins on their inner curvilinear surface can be used for selective capture of cells under flow conditions.

Original languageEnglish
Article number1603923
Number of pages7
JournalSmall
Volume13
Issue number17
DOIs
Publication statusPublished - 3 May 2017

Keywords

  • CIRCULATING TUMOR-CELLS
  • DIRECTED IMMOBILIZATION
  • MICROARRAYS
  • GENERATION
  • PLATFORM
  • CAPTURE
  • DENSITY
  • DEVICES
  • BIOLOGY
  • CHIP

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