DNA-SMART: Biopatterned Polymer Film Microchannels for Selective Immobilization of Proteins and Cells

Ann-Kathrin Schneider; Pavel M. Nikolov; Stefan Giselbrecht; Christof M. Niemeyer

doi:10.1002/smll.201603923

DNA-SMART: Biopatterned Polymer Film Microchannels for Selective Immobilization of Proteins and Cells

Ann-Kathrin Schneider, Pavel M. Nikolov, Stefan Giselbrecht, Christof M. Niemeyer^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

A novel SMART module, dubbed "DNA-SMART" (DNA substrate modification and replication by thermoforming) is reported, where polymer films are premodified with single-stranded DNA capture strands, microthermo formed into 3L) structures, and postmodified with complementary DNA-protein conjugates to realize complex biologically active surfaces within microfluidic devices. As a proof of feasibility, it is demonstrated that microchannels presenting three different proteins on their inner curvilinear surface can be used for selective capture of cells under flow conditions.

Original language	English
Article number	1603923
Number of pages	7
Journal	Small
Volume	13
Issue number	17
DOIs	https://doi.org/10.1002/smll.201603923
Publication status	Published - 3 May 2017

Keywords

CIRCULATING TUMOR-CELLS
DIRECTED IMMOBILIZATION
MICROARRAYS
GENERATION
PLATFORM
CAPTURE
DENSITY
DEVICES
BIOLOGY
CHIP

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10.1002/smll.201603923

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title = "DNA-SMART: Biopatterned Polymer Film Microchannels for Selective Immobilization of Proteins and Cells",

abstract = "A novel SMART module, dubbed {"}DNA-SMART{"} (DNA substrate modification and replication by thermoforming) is reported, where polymer films are premodified with single-stranded DNA capture strands, microthermo formed into 3L) structures, and postmodified with complementary DNA-protein conjugates to realize complex biologically active surfaces within microfluidic devices. As a proof of feasibility, it is demonstrated that microchannels presenting three different proteins on their inner curvilinear surface can be used for selective capture of cells under flow conditions.",

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year = "2017",

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doi = "10.1002/smll.201603923",

language = "English",

volume = "13",

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T2 - Biopatterned Polymer Film Microchannels for Selective Immobilization of Proteins and Cells

AU - Schneider, Ann-Kathrin

AU - Nikolov, Pavel M.

AU - Giselbrecht, Stefan

AU - Niemeyer, Christof M.

PY - 2017/5/3

Y1 - 2017/5/3

N2 - A novel SMART module, dubbed "DNA-SMART" (DNA substrate modification and replication by thermoforming) is reported, where polymer films are premodified with single-stranded DNA capture strands, microthermo formed into 3L) structures, and postmodified with complementary DNA-protein conjugates to realize complex biologically active surfaces within microfluidic devices. As a proof of feasibility, it is demonstrated that microchannels presenting three different proteins on their inner curvilinear surface can be used for selective capture of cells under flow conditions.

AB - A novel SMART module, dubbed "DNA-SMART" (DNA substrate modification and replication by thermoforming) is reported, where polymer films are premodified with single-stranded DNA capture strands, microthermo formed into 3L) structures, and postmodified with complementary DNA-protein conjugates to realize complex biologically active surfaces within microfluidic devices. As a proof of feasibility, it is demonstrated that microchannels presenting three different proteins on their inner curvilinear surface can be used for selective capture of cells under flow conditions.

KW - CIRCULATING TUMOR-CELLS

KW - DIRECTED IMMOBILIZATION

KW - MICROARRAYS

KW - GENERATION

KW - PLATFORM

KW - CAPTURE

KW - DENSITY

KW - DEVICES

KW - BIOLOGY

KW - CHIP

U2 - 10.1002/smll.201603923

DO - 10.1002/smll.201603923

M3 - Article

C2 - 28224757

SN - 1613-6810

VL - 13

JO - Small

JF - Small

IS - 17

M1 - 1603923

ER -

DNA-SMART: Biopatterned Polymer Film Microchannels for Selective Immobilization of Proteins and Cells

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Keywords

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