DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death

S. Bonassi; M. Ceppi; P. Moller; A. Azqueta; M. Milic; N. Monica; G. Brunborg; R. Godschalk; G. Koppen; S.A.S. Langie; J.P. Teixeira; M. Bruzzone; J. Da Silva; D. Benedetti; D. Cavallo; C.L. Ursini; L. Giovannelli; S. Moretti; P. Riso; C. Del Bo'; P. Russo; M. Dobrzynska; I.A. Goroshinskaya; E.I. Surikova; M. Staruchova; M. Barancokova; K. Volkovova; A. Kazimirova; B. Smolkova; B. Laffon; V. Valdiglesias; S. Pastor; R. Marcos; A. Hernandez; G. Gajski; B. Spremo-Potparevic; L. Zivkovic; E. Boutet-Robinet; H. Perdry; P. Lebailly; C.L. Perez; N. Basaran; Z. Nemeth; A. Safar; M. Dusinska; A. Collins; D. Anderson; V. Andrade; C.C. Pereira; hCOMET Project

doi:10.1038/s41598-021-95976-7

DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death

S. Bonassi^*, M. Ceppi, P. Moller, A. Azqueta, M. Milic, N. Monica, G. Brunborg, R. Godschalk, G. Koppen, S.A.S. Langie, J.P. Teixeira, M. Bruzzone, J. Da Silva, D. Benedetti, D. Cavallo, C.L. Ursini, L. Giovannelli, S. Moretti, P. Riso, C. Del Bo'P. Russo, M. Dobrzynska, I.A. Goroshinskaya, E.I. Surikova, M. Staruchova, M. Barancokova, K. Volkovova, A. Kazimirova, B. Smolkova, B. Laffon, V. Valdiglesias, S. Pastor, R. Marcos, A. Hernandez, G. Gajski, B. Spremo-Potparevic, L. Zivkovic, E. Boutet-Robinet, H. Perdry, P. Lebailly, C.L. Perez, N. Basaran, Z. Nemeth, A. Safar, M. Dusinska, A. Collins, D. Anderson, V. Andrade, C.C. Pereira, hCOMET Project

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was followed-up. Kaplan-Meier analysis indicated a worse overall survival in the medium and high tertile of DNA damage (p < 0.001). The effect of DNA damage on survival was modelled according to Cox proportional hazard regression model. The adjusted hazard ratio (HR) was 1.42 (1.06-1.90) for overall mortality, and 1.94 (1.04-3.59) for diseases of the circulatory system in subjects with the highest tertile of DNA damage. The findings of this study provide epidemiological evidence encouraging the implementation of the comet assay in preventive strategies for non-communicable diseases.

Original language	English
Article number	16793
Number of pages	11
Journal	Scientific Reports
Volume	11
Issue number	1
DOIs	https://doi.org/10.1038/s41598-021-95976-7
Publication status	Published - 18 Aug 2021

Keywords

CHROMOSOMAL-ABERRATIONS
HUMAN CANCER
LYMPHOCYTES PREDICTS
FREQUENCY
VALIDATION
BIOMARKERS
HALLMARKS
ADDUCTS
REPAIR
COHORT

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1 Erratum / corrigendum / retractions

Author Correction: DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death
Bonassi, S., Ceppi, M., Moller, P., Azqueta, A., Milic, M., Neri, M., Brunborg, G., Godschalk, R., Koppen, G., Langie, S. A. S., Teixeira, J. P., Bruzzone, M., Da Silva, J., Benedetti, D., Cavallo, D., Ursini, C. L., Giovannelli, L., Moretti, S., Riso, P., Del Bo', C., & 27 othersRusso, P., Dobrzynska, M., Goroshinskaya, I. A., Surikova, E. I., Staruchova, M., Barancokova, M., Volkovova, K., Kazimirova, A., Smolkova, B., Laffon, B., Valdiglesias, V., Pastor, S., Marcos, R., Hernández, A., Gajski, G., Spremo-Potparevic, B., Zivkovic, L., Boutet-Robinet, E., Perdry, H., Lebailly, P., Perez, C. L., Basaran, N., Nemeth, Z., Safar, A., Dusinska, M., Collins, A. & hCOMET Project, 20 Sept 2021, In: Scientific Reports. 11, 1, 2 p., 19043.
Research output: Contribution to journal › Erratum / corrigendum / retractions › Academic

Open Access

Cite this

Bonassi, S., Ceppi, M., Moller, P., Azqueta, A., Milic, M., Monica, N., Brunborg, G., Godschalk, R., Koppen, G., Langie, S. A. S., Teixeira, J. P., Bruzzone, M., Da Silva, J., Benedetti, D., Cavallo, D., Ursini, C. L., Giovannelli, L., Moretti, S., Riso, P., ... hCOMET Project (2021). DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death. Scientific Reports, 11(1), Article 16793. https://doi.org/10.1038/s41598-021-95976-7

@article{5d6f7d499b8448c5ab89e76345988c3a,

title = "DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death",

abstract = "The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was followed-up. Kaplan-Meier analysis indicated a worse overall survival in the medium and high tertile of DNA damage (p < 0.001). The effect of DNA damage on survival was modelled according to Cox proportional hazard regression model. The adjusted hazard ratio (HR) was 1.42 (1.06-1.90) for overall mortality, and 1.94 (1.04-3.59) for diseases of the circulatory system in subjects with the highest tertile of DNA damage. The findings of this study provide epidemiological evidence encouraging the implementation of the comet assay in preventive strategies for non-communicable diseases.",

keywords = "CHROMOSOMAL-ABERRATIONS, HUMAN CANCER, LYMPHOCYTES PREDICTS, FREQUENCY, VALIDATION, BIOMARKERS, HALLMARKS, ADDUCTS, REPAIR, COHORT",

author = "S. Bonassi and M. Ceppi and P. Moller and A. Azqueta and M. Milic and N. Monica and G. Brunborg and R. Godschalk and G. Koppen and S.A.S. Langie and J.P. Teixeira and M. Bruzzone and {Da Silva}, J. and D. Benedetti and D. Cavallo and C.L. Ursini and L. Giovannelli and S. Moretti and P. Riso and {Del Bo'}, C. and P. Russo and M. Dobrzynska and I.A. Goroshinskaya and E.I. Surikova and M. Staruchova and M. Barancokova and K. Volkovova and A. Kazimirova and B. Smolkova and B. Laffon and V. Valdiglesias and S. Pastor and R. Marcos and A. Hernandez and G. Gajski and B. Spremo-Potparevic and L. Zivkovic and E. Boutet-Robinet and H. Perdry and P. Lebailly and C.L. Perez and N. Basaran and Z. Nemeth and A. Safar and M. Dusinska and A. Collins and D. Anderson and V. Andrade and C.C. Pereira and {hCOMET Project}",

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day = "18",

doi = "10.1038/s41598-021-95976-7",

language = "English",

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issn = "2045-2322",

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Bonassi, S, Ceppi, M, Moller, P, Azqueta, A, Milic, M, Monica, N, Brunborg, G, Godschalk, R, Koppen, G, Langie, SAS, Teixeira, JP, Bruzzone, M, Da Silva, J, Benedetti, D, Cavallo, D, Ursini, CL, Giovannelli, L, Moretti, S, Riso, P, Del Bo', C, Russo, P, Dobrzynska, M, Goroshinskaya, IA, Surikova, EI, Staruchova, M, Barancokova, M, Volkovova, K, Kazimirova, A, Smolkova, B, Laffon, B, Valdiglesias, V, Pastor, S, Marcos, R, Hernandez, A, Gajski, G, Spremo-Potparevic, B, Zivkovic, L, Boutet-Robinet, E, Perdry, H, Lebailly, P, Perez, CL, Basaran, N, Nemeth, Z, Safar, A, Dusinska, M, Collins, A, Anderson, D, Andrade, V, Pereira, CC & hCOMET Project 2021, 'DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death', Scientific Reports, vol. 11, no. 1, 16793. https://doi.org/10.1038/s41598-021-95976-7

TY - JOUR

T1 - DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death

AU - Bonassi, S.

AU - Ceppi, M.

AU - Moller, P.

AU - Azqueta, A.

AU - Milic, M.

AU - Monica, N.

AU - Brunborg, G.

AU - Godschalk, R.

AU - Koppen, G.

AU - Langie, S.A.S.

AU - Teixeira, J.P.

AU - Bruzzone, M.

AU - Da Silva, J.

AU - Benedetti, D.

AU - Cavallo, D.

AU - Ursini, C.L.

AU - Giovannelli, L.

AU - Moretti, S.

AU - Riso, P.

AU - Del Bo', C.

AU - Russo, P.

AU - Dobrzynska, M.

AU - Goroshinskaya, I.A.

AU - Surikova, E.I.

AU - Staruchova, M.

AU - Barancokova, M.

AU - Volkovova, K.

AU - Kazimirova, A.

AU - Smolkova, B.

AU - Laffon, B.

AU - Valdiglesias, V.

AU - Pastor, S.

AU - Marcos, R.

AU - Hernandez, A.

AU - Gajski, G.

AU - Spremo-Potparevic, B.

AU - Zivkovic, L.

AU - Boutet-Robinet, E.

AU - Perdry, H.

AU - Lebailly, P.

AU - Perez, C.L.

AU - Basaran, N.

AU - Nemeth, Z.

AU - Safar, A.

AU - Dusinska, M.

AU - Collins, A.

AU - Anderson, D.

AU - Andrade, V.

AU - Pereira, C.C.

AU - hCOMET Project

PY - 2021/8/18

Y1 - 2021/8/18

N2 - The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was followed-up. Kaplan-Meier analysis indicated a worse overall survival in the medium and high tertile of DNA damage (p < 0.001). The effect of DNA damage on survival was modelled according to Cox proportional hazard regression model. The adjusted hazard ratio (HR) was 1.42 (1.06-1.90) for overall mortality, and 1.94 (1.04-3.59) for diseases of the circulatory system in subjects with the highest tertile of DNA damage. The findings of this study provide epidemiological evidence encouraging the implementation of the comet assay in preventive strategies for non-communicable diseases.

AB - The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was followed-up. Kaplan-Meier analysis indicated a worse overall survival in the medium and high tertile of DNA damage (p < 0.001). The effect of DNA damage on survival was modelled according to Cox proportional hazard regression model. The adjusted hazard ratio (HR) was 1.42 (1.06-1.90) for overall mortality, and 1.94 (1.04-3.59) for diseases of the circulatory system in subjects with the highest tertile of DNA damage. The findings of this study provide epidemiological evidence encouraging the implementation of the comet assay in preventive strategies for non-communicable diseases.

KW - CHROMOSOMAL-ABERRATIONS

KW - HUMAN CANCER

KW - LYMPHOCYTES PREDICTS

KW - FREQUENCY

KW - VALIDATION

KW - BIOMARKERS

KW - HALLMARKS

KW - ADDUCTS

KW - REPAIR

KW - COHORT

U2 - 10.1038/s41598-021-95976-7

DO - 10.1038/s41598-021-95976-7

M3 - Article

C2 - 34408182

SN - 2045-2322

VL - 11

JO - Scientific Reports

JF - Scientific Reports

IS - 1

M1 - 16793

ER -

DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death

Abstract

Keywords

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Research output

Author Correction: DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death

Cite this