Distribution and sub-types of afferent fibre in the mouse urinary bladder

M. S. Rahnama'i; B. T. Biallosterski; Ph. E. V. Van Kerrebroeck; G. A. van Koeveringe; J. I. Gillespie; S. G. de Wachter

doi:10.1016/j.jchemneu.2016.10.003

Distribution and sub-types of afferent fibre in the mouse urinary bladder

M. S. Rahnama'i^*, B. T. Biallosterski, Ph. E. V. Van Kerrebroeck, G. A. van Koeveringe, J. I. Gillespie, S. G. de Wachter

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Aim: Increased afferent fibre activity contributes to pathological conditions such as the overactive bladder syndrome. Nerve fibres running near the urothelium are considered to be afferent as no efferent system has yet been described. The aim of this study was to identify sub-types of afferent nerve fibres in the mouse bladder wall based on morphological criteria and analyse regional differences.

Materials and methods: 27 bladders of six month old C57BL/6 mice were removed and tissues were processed for immunohistochemistry. Cryostat sections were cut and stained for Protein Gene Product 9.5 (PGP), calcitonin gene related polypeptide (CGRP), neurofilament (NF), vesicular acetylcholine transporter (VAChT) and neuronal nitric oxide synthase (nNOS).

Results: In the sub-urothelium, different types of afferent nerve fibre were found, i.e. immunoreactive (IR) to; CGRP, NF, VAChT, and/or nNOS. At the bladder base, the sub-urothelium was more densely innervated by CGRP-IR and VAChT-IR nerve fibres, then at the lateral wall. NF- and nNOS nerves were sparsely distributed in the sub-urothelium throughout the bladder. At the lateral wall the inner muscle is densely innervated by CGRP-IR nerve fibres. NF, VAChT and nNOS nerves were evenly distributed in the different muscle layers throughout the bladder. Nerve fibre terminals expressing CGRP and NF were found within the extra-mural ganglia at the bladder base.

Conclusions: Different types of afferent nerve fibres were identified in the sub-urothelium of the mouse bladder. At the bladder base the sub-urothelium is more densely innervated than the lateral wall by CGRP-IR and VAChT-IR afferent nerve fibres. CGRP and NF afferent nerve fibres in the muscle layer probably relay afferent input to external ganglia located near the bladder base. The identification of different afferent nerves in the sub-urothelium suggests a functional heterogeneity of the afferent nerve fibres in the urinary bladder. (C) 2016 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	1-11
Number of pages	11
Journal	Journal of Chemical Neuroanatomy
Volume	79
DOIs	https://doi.org/10.1016/j.jchemneu.2016.10.003
Publication status	Published - Jan 2017

Keywords

Mouse
Immunohistochemistry
Afferent nerves
NITRIC-OXIDE SYNTHASE
VESICULAR ACETYLCHOLINE TRANSPORTER
SUBUROTHELIAL INTERSTITIAL-CELLS
DORSAL-ROOT GANGLION
GUINEA-PIG BLADDER
VANILLOID RECEPTOR
NEUROGENIC DETRUSOR
CHOLINERGIC NEURONS
INTRAMURAL GANGLIA
PHASIC ACTIVITY

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10.1016/j.jchemneu.2016.10.003

Cite this

@article{9f9da08c7a54469ba33264baf6e2233e,

title = "Distribution and sub-types of afferent fibre in the mouse urinary bladder",

abstract = "Aim: Increased afferent fibre activity contributes to pathological conditions such as the overactive bladder syndrome. Nerve fibres running near the urothelium are considered to be afferent as no efferent system has yet been described. The aim of this study was to identify sub-types of afferent nerve fibres in the mouse bladder wall based on morphological criteria and analyse regional differences.Materials and methods: 27 bladders of six month old C57BL/6 mice were removed and tissues were processed for immunohistochemistry. Cryostat sections were cut and stained for Protein Gene Product 9.5 (PGP), calcitonin gene related polypeptide (CGRP), neurofilament (NF), vesicular acetylcholine transporter (VAChT) and neuronal nitric oxide synthase (nNOS).Results: In the sub-urothelium, different types of afferent nerve fibre were found, i.e. immunoreactive (IR) to; CGRP, NF, VAChT, and/or nNOS. At the bladder base, the sub-urothelium was more densely innervated by CGRP-IR and VAChT-IR nerve fibres, then at the lateral wall. NF- and nNOS nerves were sparsely distributed in the sub-urothelium throughout the bladder. At the lateral wall the inner muscle is densely innervated by CGRP-IR nerve fibres. NF, VAChT and nNOS nerves were evenly distributed in the different muscle layers throughout the bladder. Nerve fibre terminals expressing CGRP and NF were found within the extra-mural ganglia at the bladder base.Conclusions: Different types of afferent nerve fibres were identified in the sub-urothelium of the mouse bladder. At the bladder base the sub-urothelium is more densely innervated than the lateral wall by CGRP-IR and VAChT-IR afferent nerve fibres. CGRP and NF afferent nerve fibres in the muscle layer probably relay afferent input to external ganglia located near the bladder base. The identification of different afferent nerves in the sub-urothelium suggests a functional heterogeneity of the afferent nerve fibres in the urinary bladder. (C) 2016 Elsevier B.V. All rights reserved.",

keywords = "Mouse, Immunohistochemistry, Afferent nerves, NITRIC-OXIDE SYNTHASE, VESICULAR ACETYLCHOLINE TRANSPORTER, SUBUROTHELIAL INTERSTITIAL-CELLS, DORSAL-ROOT GANGLION, GUINEA-PIG BLADDER, VANILLOID RECEPTOR, NEUROGENIC DETRUSOR, CHOLINERGIC NEURONS, INTRAMURAL GANGLIA, PHASIC ACTIVITY",

author = "Rahnama'i, {M. S.} and Biallosterski, {B. T.} and {Van Kerrebroeck}, {Ph. E. V.} and {van Koeveringe}, {G. A.} and Gillespie, {J. I.} and {de Wachter}, {S. G.}",

year = "2017",

month = jan,

doi = "10.1016/j.jchemneu.2016.10.003",

language = "English",

volume = "79",

pages = "1--11",

journal = "Journal of Chemical Neuroanatomy",

issn = "0891-0618",

publisher = "Elsevier Science",

}

TY - JOUR

T1 - Distribution and sub-types of afferent fibre in the mouse urinary bladder

AU - Rahnama'i, M. S.

AU - Biallosterski, B. T.

AU - Van Kerrebroeck, Ph. E. V.

AU - van Koeveringe, G. A.

AU - Gillespie, J. I.

AU - de Wachter, S. G.

PY - 2017/1

Y1 - 2017/1

N2 - Aim: Increased afferent fibre activity contributes to pathological conditions such as the overactive bladder syndrome. Nerve fibres running near the urothelium are considered to be afferent as no efferent system has yet been described. The aim of this study was to identify sub-types of afferent nerve fibres in the mouse bladder wall based on morphological criteria and analyse regional differences.Materials and methods: 27 bladders of six month old C57BL/6 mice were removed and tissues were processed for immunohistochemistry. Cryostat sections were cut and stained for Protein Gene Product 9.5 (PGP), calcitonin gene related polypeptide (CGRP), neurofilament (NF), vesicular acetylcholine transporter (VAChT) and neuronal nitric oxide synthase (nNOS).Results: In the sub-urothelium, different types of afferent nerve fibre were found, i.e. immunoreactive (IR) to; CGRP, NF, VAChT, and/or nNOS. At the bladder base, the sub-urothelium was more densely innervated by CGRP-IR and VAChT-IR nerve fibres, then at the lateral wall. NF- and nNOS nerves were sparsely distributed in the sub-urothelium throughout the bladder. At the lateral wall the inner muscle is densely innervated by CGRP-IR nerve fibres. NF, VAChT and nNOS nerves were evenly distributed in the different muscle layers throughout the bladder. Nerve fibre terminals expressing CGRP and NF were found within the extra-mural ganglia at the bladder base.Conclusions: Different types of afferent nerve fibres were identified in the sub-urothelium of the mouse bladder. At the bladder base the sub-urothelium is more densely innervated than the lateral wall by CGRP-IR and VAChT-IR afferent nerve fibres. CGRP and NF afferent nerve fibres in the muscle layer probably relay afferent input to external ganglia located near the bladder base. The identification of different afferent nerves in the sub-urothelium suggests a functional heterogeneity of the afferent nerve fibres in the urinary bladder. (C) 2016 Elsevier B.V. All rights reserved.

AB - Aim: Increased afferent fibre activity contributes to pathological conditions such as the overactive bladder syndrome. Nerve fibres running near the urothelium are considered to be afferent as no efferent system has yet been described. The aim of this study was to identify sub-types of afferent nerve fibres in the mouse bladder wall based on morphological criteria and analyse regional differences.Materials and methods: 27 bladders of six month old C57BL/6 mice were removed and tissues were processed for immunohistochemistry. Cryostat sections were cut and stained for Protein Gene Product 9.5 (PGP), calcitonin gene related polypeptide (CGRP), neurofilament (NF), vesicular acetylcholine transporter (VAChT) and neuronal nitric oxide synthase (nNOS).Results: In the sub-urothelium, different types of afferent nerve fibre were found, i.e. immunoreactive (IR) to; CGRP, NF, VAChT, and/or nNOS. At the bladder base, the sub-urothelium was more densely innervated by CGRP-IR and VAChT-IR nerve fibres, then at the lateral wall. NF- and nNOS nerves were sparsely distributed in the sub-urothelium throughout the bladder. At the lateral wall the inner muscle is densely innervated by CGRP-IR nerve fibres. NF, VAChT and nNOS nerves were evenly distributed in the different muscle layers throughout the bladder. Nerve fibre terminals expressing CGRP and NF were found within the extra-mural ganglia at the bladder base.Conclusions: Different types of afferent nerve fibres were identified in the sub-urothelium of the mouse bladder. At the bladder base the sub-urothelium is more densely innervated than the lateral wall by CGRP-IR and VAChT-IR afferent nerve fibres. CGRP and NF afferent nerve fibres in the muscle layer probably relay afferent input to external ganglia located near the bladder base. The identification of different afferent nerves in the sub-urothelium suggests a functional heterogeneity of the afferent nerve fibres in the urinary bladder. (C) 2016 Elsevier B.V. All rights reserved.

KW - Mouse

KW - Immunohistochemistry

KW - Afferent nerves

KW - NITRIC-OXIDE SYNTHASE

KW - VESICULAR ACETYLCHOLINE TRANSPORTER

KW - SUBUROTHELIAL INTERSTITIAL-CELLS

KW - DORSAL-ROOT GANGLION

KW - GUINEA-PIG BLADDER

KW - VANILLOID RECEPTOR

KW - NEUROGENIC DETRUSOR

KW - CHOLINERGIC NEURONS

KW - INTRAMURAL GANGLIA

KW - PHASIC ACTIVITY

U2 - 10.1016/j.jchemneu.2016.10.003

DO - 10.1016/j.jchemneu.2016.10.003

M3 - Article

C2 - 27773630

SN - 0891-0618

VL - 79

SP - 1

EP - 11

JO - Journal of Chemical Neuroanatomy

JF - Journal of Chemical Neuroanatomy

ER -