Distribution and Penetration of Intracerebroventricularly Administered 2'OMePS Oligonucleotide in the Mouse Brain

João Casaca-Carreira, Yasin Temel, Inaki Larrakoetxea, Ali Jahanshahi*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Web of Science)
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Abstract

Antisense oligonucleotide (AON) therapy is emerging as a potential treatment strategy for neurodegenerative diseases, such as spinal muscular atrophy, Huntington's disease, and amyotrophic lateral sclerosis. AONs function at the cellular level by, for example, direct interference with the expression of gene products or the molecular activation of neuroprotective pathways. However, AON therapy faces a major obstacle limiting its clinical application for central nervous system (CNS) disorders: the blood-brain barrier. Systemic administration of AONs leads to rapid clearance and breakdown of its molecules in the periphery. One way to overcome this obstacle is intracerebroventricular (ICV) delivery of the therapeutics directly to cerebrospinal fluid (CSF). Given the particular molecular structure of oligonucleotides, the (pharmaco) kinetic and distribution pattern of these compounds in the brain are yet to be clarified. In this study, 2'OMePS oligonucleotide delivered through ICV into CSF reached the most key structures in the brain. The distribution of this oligonucleotide differed when comparing specific brain structures and cell groups. After 48 h post-infusion, the distribution of the oligonucleotide reached its maximum and was found intracellularly in many key brain structures. These findings help understanding the kinetic and distribution pattern of 2'OMePS oligonucleotide in the brain and will direct more rational and effective use of ICV drug delivery and unleash its full therapeutic potential in managing CNS diseases.

Original languageEnglish
Pages (from-to)4-10
Number of pages7
JournalNucleic Acid Therapeutics
Volume27
Issue number1
DOIs
Publication statusPublished - Feb 2017

Keywords

  • oligonucleotide
  • distribution
  • intracerebroventricular administration
  • mouse brain
  • CENTRAL-NERVOUS-SYSTEM
  • CEREBROSPINAL-FLUID DYNAMICS
  • ANTISENSE OLIGONUCLEOTIDES
  • INTRAVENTRICULAR-INJECTIONS
  • HUNTINGTONS-DISEASE
  • CELLULAR UPTAKE
  • OLIGODEOXYNUCLEOTIDES
  • MICE
  • DELIVERY
  • PHARMACOKINETICS

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