Distinct radiation responses after in vitro mtDNA depletion are potentially related to oxidative stress

Marike W. van Gisbergen; An M. Voets; Rianne Biemans; Roland F. Hoffmann; Marie-Jose Drittij-Reijnders; Guido R. M. M. Haenen; Irene H. Heijink; Kasper M. A. Rouschop; Ludwig J. Dubois; Philippe Lambin

doi:10.1371/journal.pone.0182508

Distinct radiation responses after in vitro mtDNA depletion are potentially related to oxidative stress

Marike W. van Gisbergen^*
, An M. Voets
, Rianne Biemans
, Roland F. Hoffmann
, Marie-Jose Drittij-Reijnders
, Guido R. M. M. Haenen
, Irene H. Heijink
, Kasper M. A. Rouschop
, Ludwig J. Dubois
, Philippe Lambin

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Several clinically used drugs are mitotoxic causing mitochondrial DNA (mtDNA) variations, and thereby influence cancer treatment response. We hypothesized that radiation responsiveness will be enhanced in cellular models with decreased mtDNA content, attributed to altered reactive oxygen species (ROS) production and antioxidant capacity. For this purpose BEAS-2B, A549, and 143B cell lines were depleted from their mtDNA (rho(0)). Overall survival after irradiation was increased (p

Original language	English
Article number	0182508
Number of pages	13
Journal	PLOS ONE
Volume	12
Issue number	8
DOIs	https://doi.org/10.1371/journal.pone.0182508
Publication status	Published - 3 Aug 2017

Keywords

MITOCHONDRIAL-DNA
SUPEROXIDE-DISMUTASE
IONIZING-RADIATION
SURVIVAL CURVES
TUMOR RESPONSE
CANCER CELLS
LUNG-CANCER
METABOLISM
ANTIOXIDANT
GENERATION

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title = "Distinct radiation responses after in vitro mtDNA depletion are potentially related to oxidative stress",

abstract = "Several clinically used drugs are mitotoxic causing mitochondrial DNA (mtDNA) variations, and thereby influence cancer treatment response. We hypothesized that radiation responsiveness will be enhanced in cellular models with decreased mtDNA content, attributed to altered reactive oxygen species (ROS) production and antioxidant capacity. For this purpose BEAS-2B, A549, and 143B cell lines were depleted from their mtDNA (rho(0)). Overall survival after irradiation was increased (p",

keywords = "MITOCHONDRIAL-DNA, SUPEROXIDE-DISMUTASE, IONIZING-RADIATION, SURVIVAL CURVES, TUMOR RESPONSE, CANCER CELLS, LUNG-CANCER, METABOLISM, ANTIOXIDANT, GENERATION",

author = "\{van Gisbergen\}, \{Marike W.\} and Voets, \{An M.\} and Rianne Biemans and Hoffmann, \{Roland F.\} and Marie-Jose Drittij-Reijnders and Haenen, \{Guido R. M. M.\} and Heijink, \{Irene H.\} and Rouschop, \{Kasper M. A.\} and Dubois, \{Ludwig J.\} and Philippe Lambin",

year = "2017",

month = aug,

day = "3",

doi = "10.1371/journal.pone.0182508",

language = "English",

volume = "12",

journal = "PLOS ONE",

issn = "1932-6203",

publisher = "Public Library of Science",

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TY - JOUR

T1 - Distinct radiation responses after in vitro mtDNA depletion are potentially related to oxidative stress

AU - van Gisbergen, Marike W.

AU - Voets, An M.

AU - Biemans, Rianne

AU - Hoffmann, Roland F.

AU - Drittij-Reijnders, Marie-Jose

AU - Haenen, Guido R. M. M.

AU - Heijink, Irene H.

AU - Rouschop, Kasper M. A.

AU - Dubois, Ludwig J.

AU - Lambin, Philippe

PY - 2017/8/3

Y1 - 2017/8/3

N2 - Several clinically used drugs are mitotoxic causing mitochondrial DNA (mtDNA) variations, and thereby influence cancer treatment response. We hypothesized that radiation responsiveness will be enhanced in cellular models with decreased mtDNA content, attributed to altered reactive oxygen species (ROS) production and antioxidant capacity. For this purpose BEAS-2B, A549, and 143B cell lines were depleted from their mtDNA (rho(0)). Overall survival after irradiation was increased (p

AB - Several clinically used drugs are mitotoxic causing mitochondrial DNA (mtDNA) variations, and thereby influence cancer treatment response. We hypothesized that radiation responsiveness will be enhanced in cellular models with decreased mtDNA content, attributed to altered reactive oxygen species (ROS) production and antioxidant capacity. For this purpose BEAS-2B, A549, and 143B cell lines were depleted from their mtDNA (rho(0)). Overall survival after irradiation was increased (p

KW - MITOCHONDRIAL-DNA

KW - SUPEROXIDE-DISMUTASE

KW - IONIZING-RADIATION

KW - SURVIVAL CURVES

KW - TUMOR RESPONSE

KW - CANCER CELLS

KW - LUNG-CANCER

KW - METABOLISM

KW - ANTIOXIDANT

KW - GENERATION

U2 - 10.1371/journal.pone.0182508

DO - 10.1371/journal.pone.0182508

M3 - Article

C2 - 28771582

SN - 1932-6203

VL - 12

JO - PLOS ONE

JF - PLOS ONE

IS - 8

M1 - 0182508

ER -

Distinct radiation responses after in vitro mtDNA depletion are potentially related to oxidative stress

Abstract

Keywords

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