Distinct radiation responses after in vitro mtDNA depletion are potentially related to oxidative stress

Marike W. van Gisbergen*, An M. Voets, Rianne Biemans, Roland F. Hoffmann, Marie-Jose Drittij-Reijnders, Guido R. M. M. Haenen, Irene H. Heijink, Kasper M. A. Rouschop, Ludwig J. Dubois, Philippe Lambin

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

11 Citations (Web of Science)

Abstract

Several clinically used drugs are mitotoxic causing mitochondrial DNA (mtDNA) variations, and thereby influence cancer treatment response. We hypothesized that radiation responsiveness will be enhanced in cellular models with decreased mtDNA content, attributed to altered reactive oxygen species (ROS) production and antioxidant capacity. For this purpose BEAS-2B, A549, and 143B cell lines were depleted from their mtDNA (rho(0)). Overall survival after irradiation was increased (p

Original languageEnglish
Article number0182508
Number of pages13
JournalPLOS ONE
Volume12
Issue number8
DOIs
Publication statusPublished - 3 Aug 2017

Keywords

  • MITOCHONDRIAL-DNA
  • SUPEROXIDE-DISMUTASE
  • IONIZING-RADIATION
  • SURVIVAL CURVES
  • TUMOR RESPONSE
  • CANCER CELLS
  • LUNG-CANCER
  • METABOLISM
  • ANTIOXIDANT
  • GENERATION

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