Distal, not proximal, colonic acetate infusions promote fat oxidation and improve metabolic markers in overweight/obese men

Kirsten van der Beek, Emanuel E. Canfora*, Kaatje Lenaerts, Freddy J. Troost, Steven Olde Damink, Jens J. Holst, Ad A. M. Masclee, Cornelis H. C. Dejong, Ellen E. Blaak

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


Gut microbial-derived short-chain fatty acids (SCFA) are believed to affect host metabolism and cardiometabolic risk factors. The present study aim was to investigate the effects of proximal and distal colonic infusions with the SCFA acetate on fat oxidation and other metabolic parameters in men. In this randomized, double-blind crossover trial, six overweight/obese men [body mass index (BMI) 25-35 kg/m(2)] underwent two experimental periods: one with distal and one with proximal colonic sodium acetate infusions. A feeding catheter was endoscopically positioned at the beginning of each period and remained in the colon for three consecutive test days, enabling colonic acetate (100 or 180 mmol/l) or placebo infusion during fasting conditions and after an oral glucose load (postprandial). Fat oxidation and energy expenditure were measured using an open-circuit ventilated hood system and blood samples were repeatedly collected for 2 h during fasting and postprandial conditions. Distal colonic 180 mmol/l acetate infusions increased fasting fat oxidation (1.78 +/--0.28 compared with -0.78 +/--0.89 g fat 2 h(-1), P=0.015), fasting peptide YY (PYY, P=0.01) and postprandial glucose and insulin concentrations (P
Original languageEnglish
Pages (from-to)2073-2082
JournalClinical Science
Issue number22
Publication statusPublished - 1 Nov 2016


  • fatty acid oxidation
  • intestinal hormones
  • metabolic control
  • obesity
  • short-chain fatty acids

Cite this