Differential NF-kappaB pathways induction by Lactobacillus plantarum in the duodenum of healthy humans correlating with immune tolerance.

P. van Baarlen; F.J. Troost; S. van Hemert; C. van der Meer; W.M. de Vos; P.J. de Groot; G.J. Hooiveld; R.J. Brummer; M. Kleerebezem

doi:10.1073/pnas.0809919106

Differential NF-kappaB pathways induction by Lactobacillus plantarum in the duodenum of healthy humans correlating with immune tolerance.

P. van Baarlen, F.J. Troost, S. van Hemert, C. van der Meer, W.M. de Vos, P.J. de Groot, G.J. Hooiveld, R.J. Brummer, M. Kleerebezem^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

How do we acquire immune tolerance against food microorganisms and commensal bacteria that constitute the intestinal microbiota? We investigated this by stimulating the immune system of adults with commensal Lactobacillus plantarum bacteria. We studied the in vivo human responses to L. plantarum in a randomized double-blind placebo-controlled cross-over study. Healthy adults ingested preparations of living and heat-killed L. plantarum bacteria. Biopsies were taken from the intestinal duodenal mucosa and altered expression profiles were analyzed using whole-genome microarrays and by biological pathway reconstructions. Expression profiles of human mucosa displayed striking differences in modulation of NF-kappaB-dependent pathways, notably after consumption of living L. plantarum bacteria in different growth phases. Our in vivo study identified mucosal gene expression patterns and cellular pathways that correlated with the establishment of immune tolerance in healthy adults.

Original language	English
Pages (from-to)	2371-2376
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	106
Issue number	7
DOIs	https://doi.org/10.1073/pnas.0809919106
Publication status	Published - 1 Jan 2009

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10.1073/pnas.0809919106

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van Baarlen, P., Troost, F. J., van Hemert, S., van der Meer, C., de Vos, W. M., de Groot, P. J., Hooiveld, G. J., Brummer, R. J., & Kleerebezem, M. (2009). Differential NF-kappaB pathways induction by Lactobacillus plantarum in the duodenum of healthy humans correlating with immune tolerance. Proceedings of the National Academy of Sciences of the United States of America, 106(7), 2371-2376. https://doi.org/10.1073/pnas.0809919106

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title = "Differential NF-kappaB pathways induction by Lactobacillus plantarum in the duodenum of healthy humans correlating with immune tolerance.",

abstract = "How do we acquire immune tolerance against food microorganisms and commensal bacteria that constitute the intestinal microbiota? We investigated this by stimulating the immune system of adults with commensal Lactobacillus plantarum bacteria. We studied the in vivo human responses to L. plantarum in a randomized double-blind placebo-controlled cross-over study. Healthy adults ingested preparations of living and heat-killed L. plantarum bacteria. Biopsies were taken from the intestinal duodenal mucosa and altered expression profiles were analyzed using whole-genome microarrays and by biological pathway reconstructions. Expression profiles of human mucosa displayed striking differences in modulation of NF-kappaB-dependent pathways, notably after consumption of living L. plantarum bacteria in different growth phases. Our in vivo study identified mucosal gene expression patterns and cellular pathways that correlated with the establishment of immune tolerance in healthy adults.",

author = "{van Baarlen}, P. and F.J. Troost and {van Hemert}, S. and {van der Meer}, C. and {de Vos}, W.M. and {de Groot}, P.J. and G.J. Hooiveld and R.J. Brummer and M. Kleerebezem",

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language = "English",

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van Baarlen, P, Troost, FJ, van Hemert, S, van der Meer, C, de Vos, WM, de Groot, PJ, Hooiveld, GJ, Brummer, RJ & Kleerebezem, M 2009, 'Differential NF-kappaB pathways induction by Lactobacillus plantarum in the duodenum of healthy humans correlating with immune tolerance.', Proceedings of the National Academy of Sciences of the United States of America, vol. 106, no. 7, pp. 2371-2376. https://doi.org/10.1073/pnas.0809919106

Differential NF-kappaB pathways induction by Lactobacillus plantarum in the duodenum of healthy humans correlating with immune tolerance. / van Baarlen, P.; Troost, F.J.; van Hemert, S. et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, No. 7, 01.01.2009, p. 2371-2376.

Research output: Contribution to journal › Article › Academic › peer-review

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