Differential Mitochondrial Gene Expression in Adipose Tissue Following Weight Loss Induced by Diet or Bariatric Surgery

B.W. van der Kolk; M. Muniandy; D. Kaminska; M. Alvarez; A. Ko; Z. Miao; A. Valsesia; D. Langin; M. Vaittinen; M. Paakkonen; R. Jokinen; S. Kaye; S. Heinonen; K.A. Virtanen; D.P. Andersson; V. Mannisto; W.H. Saris; A. Astrup; M. Ryden; E.E. Blaak; P. Pajukanta; J. Pihlajamaki; K.H. Pietilainen

doi:10.1210/clinem/dgab072

Differential Mitochondrial Gene Expression in Adipose Tissue Following Weight Loss Induced by Diet or Bariatric Surgery

B.W. van der Kolk^*, M. Muniandy, D. Kaminska, M. Alvarez, A. Ko^*, Z. Miao, A. Valsesia, D. Langin, M. Vaittinen, M. Paakkonen, R. Jokinen, S. Kaye, S. Heinonen, K.A. Virtanen, D.P. Andersson, V. Mannisto, W.H. Saris, A. Astrup, M. Ryden, E.E. BlaakP. Pajukanta, J. Pihlajamaki, K.H. Pietilainen

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

6 Citations (Web of Science)

Abstract

Context: Mitochondria are essential for cellular energy homeostasis, yet their role in subcutaneous adipose tissue (SAT) during different types of weight-loss interventions remains unknown.

Objective: To investigate how SAT mitochondria change following diet-induced and bariatric surgery-induced weight-loss interventions in 4 independent weight-loss studies.

Methods: The DiOGenes study is a European multicenter dietary intervention with an 8-week low caloric diet (LCD; 800 kcal/d; n = 261) and 6-month weight-maintenance (n = 121) period. The Kuopio Obesity Surgery study (KOBS) is a Roux-en-Y gastric bypass (RYGB) surgery study (n = 172) with a 1-year follow-up. We associated weight-loss percentage with global and 2210 mitochondria-related RNA transcripts in linear regression analysis adjusted for age and sex. We repeated these analyses in 2 studies. The Finnish CRYO study has a 6-week LCD (800-1000 kcal/d; n = 19) and a 10.5-month follow-up. The Swedish DEOSH study is a RYGB surgery study with a 2-year (n = 49) and 5-year (n = 37) follow-up.

Results: Diet-induced weight loss led to a significant transcriptional downregulation of oxidative phosphorylation (DiOGenes; ingenuity pathway analysis [IPA] z-scores: -8.7 following LCD, -4.4 following weight maintenance; CRYO: IPA z-score: -5.6, all P < 0.001), while upregulation followed surgery-induced weight loss (KOBS: IPA z-score: 1.8, P < 0.001; in DEOSH: IPA z-scores: 4.0 following 2 years, 0.0 following 5 years). We confirmed an upregulated oxidative phosphorylation at the proteomics level following surgery (IPA z-score: 3.2, P < 0.001).

Conclusions: Differentially regulated SAT mitochondria-related gene expressions suggest qualitative alterations between weight-loss interventions, providing insights into the potential molecular mechanistic targets for weight-loss success.

Original language	English
Pages (from-to)	1312-1324
Number of pages	13
Journal	Journal of Clinical Endocrinology & Metabolism
Volume	106
Issue number	5
DOIs	https://doi.org/10.1210/clinem/dgab072
Publication status	Published - 1 May 2021

Keywords

BIOGENESIS
FAT
MAINTENANCE
MECHANISMS LINKING OBESITY
OVERWEIGHT
Y GASTRIC BYPASS
adipose tissue
bariatric surgery
diet-induced
mitochondria
transcriptomics
weight loss
LIFE-STYLE
INTERVENTION

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10.1210/clinem/dgab072Licence: CC BY

Cite this

van der Kolk, B. W., Muniandy, M., Kaminska, D., Alvarez, M., Ko, A., Miao, Z., Valsesia, A., Langin, D., Vaittinen, M., Paakkonen, M., Jokinen, R., Kaye, S., Heinonen, S., Virtanen, K. A., Andersson, D. P., Mannisto, V., Saris, W. H., Astrup, A., Ryden, M., ... Pietilainen, K. H. (2021). Differential Mitochondrial Gene Expression in Adipose Tissue Following Weight Loss Induced by Diet or Bariatric Surgery. Journal of Clinical Endocrinology & Metabolism, 106(5), 1312-1324. https://doi.org/10.1210/clinem/dgab072

@article{e492391243cb44c3bd4c2c0d424d3aa5,

title = "Differential Mitochondrial Gene Expression in Adipose Tissue Following Weight Loss Induced by Diet or Bariatric Surgery",

abstract = "Context: Mitochondria are essential for cellular energy homeostasis, yet their role in subcutaneous adipose tissue (SAT) during different types of weight-loss interventions remains unknown.Objective: To investigate how SAT mitochondria change following diet-induced and bariatric surgery-induced weight-loss interventions in 4 independent weight-loss studies.Methods: The DiOGenes study is a European multicenter dietary intervention with an 8-week low caloric diet (LCD; 800 kcal/d; n = 261) and 6-month weight-maintenance (n = 121) period. The Kuopio Obesity Surgery study (KOBS) is a Roux-en-Y gastric bypass (RYGB) surgery study (n = 172) with a 1-year follow-up. We associated weight-loss percentage with global and 2210 mitochondria-related RNA transcripts in linear regression analysis adjusted for age and sex. We repeated these analyses in 2 studies. The Finnish CRYO study has a 6-week LCD (800-1000 kcal/d; n = 19) and a 10.5-month follow-up. The Swedish DEOSH study is a RYGB surgery study with a 2-year (n = 49) and 5-year (n = 37) follow-up.Results: Diet-induced weight loss led to a significant transcriptional downregulation of oxidative phosphorylation (DiOGenes; ingenuity pathway analysis [IPA] z-scores: -8.7 following LCD, -4.4 following weight maintenance; CRYO: IPA z-score: -5.6, all P < 0.001), while upregulation followed surgery-induced weight loss (KOBS: IPA z-score: 1.8, P < 0.001; in DEOSH: IPA z-scores: 4.0 following 2 years, 0.0 following 5 years). We confirmed an upregulated oxidative phosphorylation at the proteomics level following surgery (IPA z-score: 3.2, P < 0.001).Conclusions: Differentially regulated SAT mitochondria-related gene expressions suggest qualitative alterations between weight-loss interventions, providing insights into the potential molecular mechanistic targets for weight-loss success.",

keywords = "BIOGENESIS, FAT, MAINTENANCE, MECHANISMS LINKING OBESITY, OVERWEIGHT, Y GASTRIC BYPASS, adipose tissue, bariatric surgery, diet-induced, mitochondria, transcriptomics, weight loss, LIFE-STYLE, INTERVENTION",

author = "{van der Kolk}, B.W. and M. Muniandy and D. Kaminska and M. Alvarez and A. Ko and Z. Miao and A. Valsesia and D. Langin and M. Vaittinen and M. Paakkonen and R. Jokinen and S. Kaye and S. Heinonen and K.A. Virtanen and D.P. Andersson and V. Mannisto and W.H. Saris and A. Astrup and M. Ryden and E.E. Blaak and P. Pajukanta and J. Pihlajamaki and K.H. Pietilainen",

year = "2021",

month = may,

day = "1",

doi = "10.1210/clinem/dgab072",

language = "English",

volume = "106",

pages = "1312--1324",

journal = "Journal of Clinical Endocrinology & Metabolism",

issn = "0021-972X",

publisher = "Oxford University Press",

number = "5",

}

van der Kolk, BW, Muniandy, M, Kaminska, D, Alvarez, M, Ko, A, Miao, Z, Valsesia, A, Langin, D, Vaittinen, M, Paakkonen, M, Jokinen, R, Kaye, S, Heinonen, S, Virtanen, KA, Andersson, DP, Mannisto, V, Saris, WH, Astrup, A, Ryden, M, Blaak, EE, Pajukanta, P, Pihlajamaki, J & Pietilainen, KH 2021, 'Differential Mitochondrial Gene Expression in Adipose Tissue Following Weight Loss Induced by Diet or Bariatric Surgery', Journal of Clinical Endocrinology & Metabolism, vol. 106, no. 5, pp. 1312-1324. https://doi.org/10.1210/clinem/dgab072

TY - JOUR

T1 - Differential Mitochondrial Gene Expression in Adipose Tissue Following Weight Loss Induced by Diet or Bariatric Surgery

AU - van der Kolk, B.W.

AU - Muniandy, M.

AU - Kaminska, D.

AU - Alvarez, M.

AU - Ko, A.

AU - Miao, Z.

AU - Valsesia, A.

AU - Langin, D.

AU - Vaittinen, M.

AU - Paakkonen, M.

AU - Jokinen, R.

AU - Kaye, S.

AU - Heinonen, S.

AU - Virtanen, K.A.

AU - Andersson, D.P.

AU - Mannisto, V.

AU - Saris, W.H.

AU - Astrup, A.

AU - Ryden, M.

AU - Blaak, E.E.

AU - Pajukanta, P.

AU - Pihlajamaki, J.

AU - Pietilainen, K.H.

PY - 2021/5/1

Y1 - 2021/5/1

N2 - Context: Mitochondria are essential for cellular energy homeostasis, yet their role in subcutaneous adipose tissue (SAT) during different types of weight-loss interventions remains unknown.Objective: To investigate how SAT mitochondria change following diet-induced and bariatric surgery-induced weight-loss interventions in 4 independent weight-loss studies.Methods: The DiOGenes study is a European multicenter dietary intervention with an 8-week low caloric diet (LCD; 800 kcal/d; n = 261) and 6-month weight-maintenance (n = 121) period. The Kuopio Obesity Surgery study (KOBS) is a Roux-en-Y gastric bypass (RYGB) surgery study (n = 172) with a 1-year follow-up. We associated weight-loss percentage with global and 2210 mitochondria-related RNA transcripts in linear regression analysis adjusted for age and sex. We repeated these analyses in 2 studies. The Finnish CRYO study has a 6-week LCD (800-1000 kcal/d; n = 19) and a 10.5-month follow-up. The Swedish DEOSH study is a RYGB surgery study with a 2-year (n = 49) and 5-year (n = 37) follow-up.Results: Diet-induced weight loss led to a significant transcriptional downregulation of oxidative phosphorylation (DiOGenes; ingenuity pathway analysis [IPA] z-scores: -8.7 following LCD, -4.4 following weight maintenance; CRYO: IPA z-score: -5.6, all P < 0.001), while upregulation followed surgery-induced weight loss (KOBS: IPA z-score: 1.8, P < 0.001; in DEOSH: IPA z-scores: 4.0 following 2 years, 0.0 following 5 years). We confirmed an upregulated oxidative phosphorylation at the proteomics level following surgery (IPA z-score: 3.2, P < 0.001).Conclusions: Differentially regulated SAT mitochondria-related gene expressions suggest qualitative alterations between weight-loss interventions, providing insights into the potential molecular mechanistic targets for weight-loss success.

AB - Context: Mitochondria are essential for cellular energy homeostasis, yet their role in subcutaneous adipose tissue (SAT) during different types of weight-loss interventions remains unknown.Objective: To investigate how SAT mitochondria change following diet-induced and bariatric surgery-induced weight-loss interventions in 4 independent weight-loss studies.Methods: The DiOGenes study is a European multicenter dietary intervention with an 8-week low caloric diet (LCD; 800 kcal/d; n = 261) and 6-month weight-maintenance (n = 121) period. The Kuopio Obesity Surgery study (KOBS) is a Roux-en-Y gastric bypass (RYGB) surgery study (n = 172) with a 1-year follow-up. We associated weight-loss percentage with global and 2210 mitochondria-related RNA transcripts in linear regression analysis adjusted for age and sex. We repeated these analyses in 2 studies. The Finnish CRYO study has a 6-week LCD (800-1000 kcal/d; n = 19) and a 10.5-month follow-up. The Swedish DEOSH study is a RYGB surgery study with a 2-year (n = 49) and 5-year (n = 37) follow-up.Results: Diet-induced weight loss led to a significant transcriptional downregulation of oxidative phosphorylation (DiOGenes; ingenuity pathway analysis [IPA] z-scores: -8.7 following LCD, -4.4 following weight maintenance; CRYO: IPA z-score: -5.6, all P < 0.001), while upregulation followed surgery-induced weight loss (KOBS: IPA z-score: 1.8, P < 0.001; in DEOSH: IPA z-scores: 4.0 following 2 years, 0.0 following 5 years). We confirmed an upregulated oxidative phosphorylation at the proteomics level following surgery (IPA z-score: 3.2, P < 0.001).Conclusions: Differentially regulated SAT mitochondria-related gene expressions suggest qualitative alterations between weight-loss interventions, providing insights into the potential molecular mechanistic targets for weight-loss success.

KW - BIOGENESIS

KW - FAT

KW - MAINTENANCE

KW - MECHANISMS LINKING OBESITY

KW - OVERWEIGHT

KW - Y GASTRIC BYPASS

KW - adipose tissue

KW - bariatric surgery

KW - diet-induced

KW - mitochondria

KW - transcriptomics

KW - weight loss

KW - LIFE-STYLE

KW - INTERVENTION

U2 - 10.1210/clinem/dgab072

DO - 10.1210/clinem/dgab072

M3 - Article

C2 - 33560372

SN - 0021-972X

VL - 106

SP - 1312

EP - 1324

JO - Journal of Clinical Endocrinology & Metabolism

JF - Journal of Clinical Endocrinology & Metabolism

IS - 5

ER -