Differential Expression Profile of miR-27b, miR-29a and miR-155 in Chronic Lymphocytic Leukemia and Breast Cancer Patients

Farzaneh Raeisi, Esmaeil Mahmoudi Mahmoudi, Mina Dehghani-Samani, Seyedeh Sahar Ebrahimi Hosseini, Ameneh Mehri Ghahfarrokhi, Asghar Arshi, Kayvan Forghanparast, Samaneh Ghazanfari

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Over the past decade, studies on microRNA (miRNA) and cancer quickly became known. miRNAs are small non-coding RNAs that play a vital role in regulation of gene expression. In the present study, the expression of miR-27b, miR-29a, and miR-155, their prognostic roles, and their potential targets in chronic lymphocytic leukemia (CLL) and breast cancer (BC) by qRT-PCR were investigated. In two case-control studies, qRT-PCR was used to analyze the peripheral blood serum of 15 CLL patients and tissue samples of 15 BC patients for the expression of miR-27b, miR-29a, and miR-155. miRNA expression levels were calculated using the qRT-PCR method. The results revealed a significant increase in the expression of all miRNAs in patients with BC and CLL compared with respective healthy groups (p <0.001). In BC patients, there was a significant difference between the expression of miR-155 and miR-29a (p <0.05), miR-155 and miR-27b (p <0.01), and miR-27b and miR-29a (p <0.001). In CLL patients, a significant difference between expression of both miR-27b and miR-29a compared with expression of miR-155 (p <0.001) was found. Furthermore, a significant association between miR-155 and prevascular invasion was found. Significantly, elevated circulating miRNAs were shown to be BC specific and could differentiate BC tissues from the controls. It was demonstrated that miRNAs used in this study and their expression profiles can be developed as biomarkers for early diagnosis and prognosis of CLL and BC. Further studies utilizing a larger test group of patients would provide identification of miRNAs as key players in intercellular interactions.

Original languageEnglish
Pages (from-to)230-237
Number of pages8
JournalMolecular Therapy - Oncolytics
Volume16
DOIs
Publication statusPublished - 27 Mar 2020

Keywords

  • BIOMARKERS
  • MICRORNAS
  • PCR
  • TARGETS

Cite this

Raeisi, F., Mahmoudi, E. M., Dehghani-Samani, M., Ebrahimi Hosseini, S. S., Ghahfarrokhi, A. M., Arshi, A., Forghanparast, K., & Ghazanfari, S. (2020). Differential Expression Profile of miR-27b, miR-29a and miR-155 in Chronic Lymphocytic Leukemia and Breast Cancer Patients. Molecular Therapy - Oncolytics, 16, 230-237. https://doi.org/10.1016/j.omto.2020.01.004