Diagnostic accuracy for apical and chronic periodontitis biomarkers in gingival crevicular fluid: an exploratory study

Mauricio Baeza, Mauricio Garrido, Patricia Hernandez-Rios, Andrea Dezerega, Jocelyn Garcia-Sesnich, Franz Strauss, Juan Pablo Aitken, Emmanuel Lesaffre, Sophie Vanbelle, Jorge Gamonal, Romina Brignardello-Petersen, Taina Tervahartiala, Timo Sorsa, Marcela Hernandez*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


Aim The aim of this study was to assess the levels and diagnostic accuracy of a set of potential biomarkers of periodontal tissue metabolism in gingival crevicular fluid (GCF) from patients with chronic periodontitis (CP) and asymptomatic apical periodontitis ( AAP). Materials and MethodsThirty one GCF samples from 11 CP patients, 44 GCF samples from 38 AAP patients and 31 GCF samples from 13 healthy volunteers were obtained (N=106). Matrix metalloproteinases (MMPs) -2 and -9 were determined by zymography; levels of MMP-8 by ELISA and IFMA and MPO by ELISA. IL-1, IL-6, TNF, DKK-1, Osteonectin, Periostin, TRAP-5 and OPG were determined by a multiplex quantitative panel. Statistical analysis was performed using linear mixed-effects models. ResultsThe MMP-9 and MMP-8 were higher in CP, followed by AAP, versus healthy individuals (p0.97) in CP, and for the active form of MMP-9 and MMP-8 (AUC>0.90) in AAP. ConclusionGingival crevicular fluid composition is modified by CP and AAP. MMP-9 and MMP-8 show diagnostic potential for CP and AAP, whereas MMP-2 and TRAP-5 are useful only for CP.
Original languageEnglish
Pages (from-to)34-45
JournalJournal of Clinical Periodontology
Issue number1
Publication statusPublished - Jan 2016


  • asymptomatic apical periodontitis
  • biomarkers
  • chronic periodontitis
  • gingival crevicular fluid
  • sensitivity
  • specificity

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