TY - JOUR
T1 - Diagnostic accuracy for apical and chronic periodontitis biomarkers in gingival crevicular fluid: an exploratory study
AU - Baeza, Mauricio
AU - Garrido, Mauricio
AU - Hernandez-Rios, Patricia
AU - Dezerega, Andrea
AU - Garcia-Sesnich, Jocelyn
AU - Strauss, Franz
AU - Aitken, Juan Pablo
AU - Lesaffre, Emmanuel
AU - Vanbelle, Sophie
AU - Gamonal, Jorge
AU - Brignardello-Petersen, Romina
AU - Tervahartiala, Taina
AU - Sorsa, Timo
AU - Hernandez, Marcela
PY - 2016/1
Y1 - 2016/1
N2 - Aim The aim of this study was to assess the levels and diagnostic accuracy of a set of potential biomarkers of periodontal tissue metabolism in gingival crevicular fluid (GCF) from patients with chronic periodontitis (CP) and asymptomatic apical periodontitis ( AAP). Materials and MethodsThirty one GCF samples from 11 CP patients, 44 GCF samples from 38 AAP patients and 31 GCF samples from 13 healthy volunteers were obtained (N=106). Matrix metalloproteinases (MMPs) -2 and -9 were determined by zymography; levels of MMP-8 by ELISA and IFMA and MPO by ELISA. IL-1, IL-6, TNF, DKK-1, Osteonectin, Periostin, TRAP-5 and OPG were determined by a multiplex quantitative panel. Statistical analysis was performed using linear mixed-effects models. ResultsThe MMP-9 and MMP-8 were higher in CP, followed by AAP, versus healthy individuals (p0.97) in CP, and for the active form of MMP-9 and MMP-8 (AUC>0.90) in AAP. ConclusionGingival crevicular fluid composition is modified by CP and AAP. MMP-9 and MMP-8 show diagnostic potential for CP and AAP, whereas MMP-2 and TRAP-5 are useful only for CP.
AB - Aim The aim of this study was to assess the levels and diagnostic accuracy of a set of potential biomarkers of periodontal tissue metabolism in gingival crevicular fluid (GCF) from patients with chronic periodontitis (CP) and asymptomatic apical periodontitis ( AAP). Materials and MethodsThirty one GCF samples from 11 CP patients, 44 GCF samples from 38 AAP patients and 31 GCF samples from 13 healthy volunteers were obtained (N=106). Matrix metalloproteinases (MMPs) -2 and -9 were determined by zymography; levels of MMP-8 by ELISA and IFMA and MPO by ELISA. IL-1, IL-6, TNF, DKK-1, Osteonectin, Periostin, TRAP-5 and OPG were determined by a multiplex quantitative panel. Statistical analysis was performed using linear mixed-effects models. ResultsThe MMP-9 and MMP-8 were higher in CP, followed by AAP, versus healthy individuals (p0.97) in CP, and for the active form of MMP-9 and MMP-8 (AUC>0.90) in AAP. ConclusionGingival crevicular fluid composition is modified by CP and AAP. MMP-9 and MMP-8 show diagnostic potential for CP and AAP, whereas MMP-2 and TRAP-5 are useful only for CP.
KW - asymptomatic apical periodontitis
KW - biomarkers
KW - chronic periodontitis
KW - gingival crevicular fluid
KW - sensitivity
KW - specificity
U2 - 10.1111/jcpe.12479
DO - 10.1111/jcpe.12479
M3 - Article
C2 - 26556177
SN - 0303-6979
VL - 43
SP - 34
EP - 45
JO - Journal of Clinical Periodontology
JF - Journal of Clinical Periodontology
IS - 1
ER -