Seersucker is a thin puckered woven fabric in which some threads bunched together, giving a wrinkled appearance in place. Conventionally seersucker fabric is produced on weaving machines with some special warp tensioning devices. The aim of this work was to develop knitted seersucker fabric on single cylinder weft knitting machine using plain jersey structure. Core spun cotton yarn and cotton plain yarn of same linear density were fed at regular intervals to the feeders on the machine. The produced sample has a regular puckering stripe along the width of the fabric with same height. Air permeability and moisture management tests of wet processed samples were performed. The results indicated that the knitted seersucker fabric has good comfort properties as well as better aesthetic appearance.