Abstract
In this article we report on the development and optimization of a biomimetic sensor for the label free detection of the amino acid sarcosine, a molecule which is seen as a biomarker for prostate cancer. The recognition elements were sarcosine-imprinted poly-aminothiophenol (p-ATP) layers deposited by electro-polymerization onto screen-printed gold electrodes and, for comparison, onto carbon electrodes covered first with a gold-nanoparticles interlayer. Using the latter type of electrodes, we reached a detection limit below 1 nM in aqueous buffer solutions with an accessible concentration range from the nano to the micromolar scale. This was achieved by a careful thickness optimization of the Au-nanoparticle and the p-ATP layers in combination with Faradaic impedance spectroscopy as a readout method. The sensor showed an excellent reproducibility, a good stability with time, and a surprisingly low cross-selectivity towards other proteins. 2017 Elsevier B.V. All rights reserved.
Original language | English |
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Pages (from-to) | 461-470 |
Number of pages | 10 |
Journal | Sensors and Actuators B-Chemical |
Volume | 246 |
DOIs | |
Publication status | Published - Jul 2017 |
Externally published | Yes |
Keywords
- Molecularly imprinted polymers
- Cold nanoparticles
- Faradaic electro-chemical Impedance
- spectroscopy EIS
- Sarcosine
- Prostate cancer
- ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY
- PROSTATE-CANCER DETECTION
- SCREEN-PRINTED ELECTRODE
- HUMAN SERUM
- IMMUNOSENSOR
- BIOSENSOR
- URINE
- MARKER
- CHROMATOGRAPHY
- NANOPARTICLES