Development, Characterization and In Vitro Biological Properties of Scaffolds Fabricated From Calcium Phosphate Nanoparticles

Lizette Morejón*, José Angel Delgado, Alexandre Antunes Ribeiro, Marize Varella de Oliveira, Eduardo Mendizábal, Ibrahim García, Adrián Alfonso, Patrina Poh, Martijn van Griensven, Elizabeth R Balmayor*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

24 Citations (Web of Science)


Ceramic materials mimic the mineral composition of native bone and feature osteoconductive properties; they are therefore used to regenerate bone tissue. Much research focuses on increasing the porosity and pore interconnectivity of ceramic scaffolds to increase osteoconductivity, cell migration and cell-cell interaction. We aimed to fabricate biocompatible 3D-scaffolds featuring macro- and microporous calcium phosphates with high pore interconnection. Nanoparticles of hydroxyapatite (HA) and calcium deficient hydroxyapatite (CDHA) were synthesized by wet chemical precipitation. Scaffolds were produced from them by the replication polymeric foam technique. Solid content and sintering temperature were varied. Nanoparticles and scaffolds were characterized regarding morphology, chemical and mineral composition, porosity and mechanical properties. Biocompatibility, cell attachment and distribution were evaluated in vitro with human adipose mesenchymal stem cells. Scaffolds with total porosity of 71%-87%, pores in the range of 280-550 µm and connectivity density up to 43 mm-3 were obtained. Smaller pore sizes were obtained at higher sintering temperature. High solid content resulted in a decrease of total porosity but increased interconnectivity. Scaffolds 50HA/50β-TCP featured superior interconnectivity and mechanical properties. They were bioactive and biocompatible. High HA solid content (40 wt.%) in the HA pure scaffolds was negative for cell viability and proliferation, while in the 50HA/50β-TCP composite scaffolds it resulted more biocompatible.

Original languageEnglish
Article number1790
Number of pages23
JournalInternational journal of molecular sciences
Issue number7
Publication statusPublished - 11 Apr 2019
Externally publishedYes


  • Biocompatible Materials/chemistry
  • Calcium Phosphates/chemistry
  • Cell Adhesion
  • Cell Survival
  • Cells, Cultured
  • Durapatite/chemistry
  • Humans
  • Materials Testing
  • Mesenchymal Stem Cells/cytology
  • Nanoparticles/chemistry
  • Porosity
  • Tissue Engineering
  • Tissue Scaffolds/chemistry
  • VIVO
  • bone tissue engineering
  • beta-tricalcium phosphate
  • biphasic calcium phosphates
  • scaffolds
  • hydroxyapatite

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