Development and application of an electron spin resonance spectrometry method for the determination of oxygen free radical formation by particulate matter

J.J. Briedé*, T.M.C.M. de Kok, J.G.F. Hogervorst, E.J.C. Moonen, C.L. op den Camp, J.C.S. Kleinjans

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


Exposure to increased levels of ambient particulate matter (PM) are associated with several health effects, including cardiopulmonary diseases. The formation of reactive oxygen species (ROS) is thought to play an important role in the induction of these health effects. To quantify the ROS generating capacityof PM,we developed an improved electron spin resonance (ESR) spectrometry-based method. ROS formation was measured directly on PM-containing filters, thereby avoiding the selective extraction of components and loss of material or reactivity, which is likely to occur during filter extraction. Also, ascorbic acid was added to stimulate ROS formation. This method was applied to PM10 samples originating from different sources. The radical generating capacity of PM10 from both gasoline and diesel engine exhaust was significantly higher as compared to that of PM10 from ambient or indoor air. Furthermore, in urban PM10 and PM2.5, ROS-generating capacity significantly correlated with concentrations of polycyclic aromatic hydrocarbon content and particular transition metals. This indicates thatthis improved ESR method may be a valuable tool for evaluating the relationship between ROS formation by PM and the adverse health effects associated with this type of air pollution
Original languageEnglish
Pages (from-to)8420-8426
JournalEnvironmental Science & Technology
Issue number21
Publication statusPublished - 1 Jan 2005

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