Determination of low molecular weight heparin in clinical laboratory

Janjaap van Putten, Marjo van de Ruit, Marlene Beunis, H. Coenraad Hemker

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    Abstract

    The applicability was investigated of automated spectrophotometric heparin assays and three clotting assays for determination of two low molecular weight (LMW) heparin fractions: Org 10172 and DxN10 and two infractionated commercially available heparins. The relative activity of the two commercially available heparins was similar in the anti-Xa assay, in the anti-IIa assay and in 3 clotting assays. The LMW heparins showed markedly different relative activity in all 5 assays. The activities of those heparin preparations relative to the standard heparin were compared in the 5 assays, but standardization against a standard heparin preparation appeared impossible. Methods of heparin determination can be used to monitor treatment with a heparin preparation only if the same preparation is used as a reference substance.
    Original languageEnglish
    Pages (from-to)205-210
    Number of pages6
    JournalHaemostasis
    Volume14
    Issue number2
    DOIs
    Publication statusPublished - 1984

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