Detection of Peptide-Based Nanoparticles in Blood Plasma by ELISA

Gerard H. Bode, Karin E. Pickl, Maria Sanchez-Purra, Berta Albaiges, Salvador Borros, Andy J. G. Poetgens, Christoph Schmitz, Frank M. Sinner, Mario Losen, Harry W. M. Steinbusch, Hans-Georg Frank, Pilar Martinez-Martinez*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Web of Science)

Abstract

Aims The aim of the current study was to develop a method to detect peptide-linked nanoparticles in blood plasma. Materials & Methods A convenient enzyme linked immunosorbent assay (ELISA) was developed for the detection of peptides functionalized with biotin and fluorescein groups. As a proof of principle, polymerized pentafluorophenyl methacrylate nanoparticles linked to biotin-carboxyfluorescein labeled peptides were intravenously injected in Wistar rats. Serial blood plasma samples were analyzed by ELISA and by liquid chromatography mass spectrometry (LC/MS) technology. Results The ELISA based method for the detection of FITC labeled peptides had a detection limit of 1 ng/mL. We were able to accurately measure peptides bound to pentafluorophenyl methacrylate nanoparticles in blood plasma of rats, and similar results were obtained by LC/MS. Conclusions We detected FITC-labeled peptides on pentafluorophenyl methacrylate nanoparticles after injection in vivo. This method can be extended to detect nanoparticles with different chemical compositions.
Original languageEnglish
Article numbere0126136
JournalPLOS ONE
Volume10
Issue number5
DOIs
Publication statusPublished - 21 May 2015

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