Detection of Merkel Cell Polyomavirus in Seborrheic Keratosis

Lisa M. Hillen, Dorit Rennspiess, Ernst-Jan Speel, Anke M. Haugg, Veronique Winnepenninckx, Axel Zur Hausen*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Web of Science)

Abstract

Seborrheic keratosis (SK) is the most common benign cutaneous neoplasm. A subset shows increased p16 expression. Since SK shares several features with verruca vulgaris, e.g., increased p16 expression, human papillomaviruses (HPV) have been suggested as possible causal agents. However, a relevant association could not be established between HPV and SK. In the present study we aimed to investigate the presence of Merkel cell polyomavirus (MCPyV) in relation to p16 expression in SK. P16 expression was investigated using immunohistochemistry (IHC). Presence of MCPyV was assessed in 23 formalin-fixed paraffin embedded tissue samples of SK by molecular techniques (i.e., PCR and FISH) and IHC. 16/23 SK showed strong to moderate p16 expression. 6/23 of SK were MCPyV positive by PCR which was confirmed by FISH. Of interest, two samples with strong FISH signals also showed MCPyV expression as tested by IHC. Samples with weaker signal intensity were negative in IHC. P16 expression was not associated with the presence of MCPyV. Concluding, the detection of MCPyV DNA by PCR and FISH in SK reflects the widespread prevalence of MCPyV in the skin. However, low detection rates exclude MCPyV as a major pathogenic factor in SK, most likely representing a coincidental infection. P16 IHC does not appear as useful adjunctive surrogate marker for the presence of MCPyV in SK.
Original languageEnglish
Article number2648
Number of pages8
JournalFrontiers in microbiology
Volume8
DOIs
Publication statusPublished - 9 Jan 2018

Keywords

  • seborrheic keratosis
  • Merkel cell polyomavirus
  • MCPyV
  • p16
  • fluorescence in situ hybridization
  • FISH
  • immunohistochemistry
  • IHC
  • IN-SITU HYBRIDIZATION
  • CERVICAL INTRAEPITHELIAL NEOPLASIA
  • IMPROVES INTEROBSERVER AGREEMENT
  • CHRONIC LYMPHOCYTIC-LEUKEMIA
  • NONMELANOMA SKIN-CANCER
  • BOWENS-DISEASE
  • T-ANTIGEN
  • PAPILLOMAVIRUS DNA
  • ACTINIC KERATOSIS
  • P16 EXPRESSION

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