Detection of Anti-neutrophil Cytoplasmic Antibodies (ANCA) by Indirect Immunofluorescence

Joyce van Beers, J. Vanderlocht, C. Roozendaal, Jan Damoiseaux*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademic


The eventual presence of anti-neutrophil cytoplasmic antibodies (ANCA) can initially be screened with indirect immunofluorescence (IIF). The majority of laboratories that facilitate ANCA testing use commercial kits. Although in-house assays are not encouraged in routine clinical laboratories, knowledge on the methodological aspects of the assay remains of importance. These aspects include choice of substrate, choice of fixative, staining procedure, and interpretation procedure. In this paper details on the methodology are provided and discussed in the context of the clinical application.
Original languageEnglish
Title of host publicationAutoantibodies
Subtitle of host publicationMethods and Protocols
EditorsGunnar Houen
PublisherHumana Press
ISBN (Electronic)978-1-4939-8949-2
ISBN (Print)978-1-4939-8948-5
Publication statusPublished - 2019

Publication series

SeriesMethods in Molecular Biology


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