The eventual presence of anti-neutrophil cytoplasmic antibodies (ANCA) can initially be screened with indirect immunofluorescence (IIF). The majority of laboratories that facilitate ANCA testing use commercial kits. Although in-house assays are not encouraged in routine clinical laboratories, knowledge on the methodological aspects of the assay remains of importance. These aspects include choice of substrate, choice of fixative, staining procedure, and interpretation procedure. In this paper details on the methodology are provided and discussed in the context of the clinical application.
|Title of host publication||Autoantibodies|
|Subtitle of host publication||Methods and Protocols|
|Publication status||Published - 2019|
|Series||Methods in Molecular Biology|
van Beers, J., Vanderlocht, J., Roozendaal, C., & Damoiseaux, J. (2019). Detection of Anti-neutrophil Cytoplasmic Antibodies (ANCA) by Indirect Immunofluorescence. In G. Houen (Ed.), Autoantibodies: Methods and Protocols (pp. 47-62). Humana Press. Methods in Molecular Biology https://doi.org/10.1007/978-1-4939-8949-2_4