Detection of Anti-neutrophil Cytoplasmic Antibodies (ANCA) by Indirect Immunofluorescence

Joyce van Beers; J. Vanderlocht; C. Roozendaal; Jan Damoiseaux

doi:10.1007/978-1-4939-8949-2_4

Detection of Anti-neutrophil Cytoplasmic Antibodies (ANCA) by Indirect Immunofluorescence

Joyce van Beers, J. Vanderlocht, C. Roozendaal, Jan Damoiseaux

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic

Abstract

The eventual presence of anti-neutrophil cytoplasmic antibodies (ANCA) can initially be screened with indirect immunofluorescence (IIF). The majority of laboratories that facilitate ANCA testing use commercial kits. Although in-house assays are not encouraged in routine clinical laboratories, knowledge on the methodological aspects of the assay remains of importance. These aspects include choice of substrate, choice of fixative, staining procedure, and interpretation procedure. In this paper details on the methodology are provided and discussed in the context of the clinical application.

Original language	English
Title of host publication	Autoantibodies
Subtitle of host publication	Methods and Protocols
Editors	Gunnar Houen
Publisher	Humana Press
Pages	47-62
ISBN (Electronic)	978-1-4939-8949-2
ISBN (Print)	978-1-4939-8948-5
DOIs	https://doi.org/10.1007/978-1-4939-8949-2_4
Publication status	Published - 2019

Publication series

Series	Methods in Molecular Biology
ISSN	1064-3745

Access to Document

10.1007/978-1-4939-8949-2_4

Cite this

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abstract = "The eventual presence of anti-neutrophil cytoplasmic antibodies (ANCA) can initially be screened with indirect immunofluorescence (IIF). The majority of laboratories that facilitate ANCA testing use commercial kits. Although in-house assays are not encouraged in routine clinical laboratories, knowledge on the methodological aspects of the assay remains of importance. These aspects include choice of substrate, choice of fixative, staining procedure, and interpretation procedure. In this paper details on the methodology are provided and discussed in the context of the clinical application.",

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