Design and performance of a dark-field probe with confocal Raman spectroscopy for ophthalmic applications

Shuo Zhang; Roel J. Erckens; Franciscus H. M. Jongsma; Carroll A. B. Webers; Tos T. J. M. Berendschot

doi:10.1002/jrs.6125

Design and performance of a dark-field probe with confocal Raman spectroscopy for ophthalmic applications

Shuo Zhang, Roel J. Erckens, Franciscus H. M. Jongsma, Carroll A. B. Webers, Tos T. J. M. Berendschot^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

A dark-field ophthalmic contact probe for analysis of aqueous humor (AH) was designed by ray tracing and constructed in quartz glass for experimental use as a prototype probe in a confocal Raman spectroscopic setup. The misalignment tolerance of this probe was theoretically evaluated, whereas the optical performance was experimentally evaluated by a concentration curve of the ocular drug phenylephrine HCl in an artificial eye model. The results indicated a ninefold improvement in misalignment tolerance and a 36-fold improvement in the signal-to-noise ratio at the phenylephrine HCl 1002 cm(-1) peak compared with a previous design. The prototype showed a good linear fit with the phenylephrine HCl concentration, with an R-2 of 0.996. Phenylephrine HCl concentration down to 0.1% is detectable.

Original language	English
Pages (from-to)	1371-1375
Number of pages	5
Journal	Journal of Raman Spectroscopy
Volume	52
Issue number	7
Early online date	5 May 2021
DOIs	https://doi.org/10.1002/jrs.6125
Publication status	Published - Jul 2021

Keywords

aqueous humor
dark&#8208
field illumination
ocular drug
Raman spectroscopy

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10.1002/jrs.6125Licence: CC BY-NC-ND

Cite this

@article{688c9fe0d24a49a0b75f0eefe8169058,

title = "Design and performance of a dark-field probe with confocal Raman spectroscopy for ophthalmic applications",

abstract = "A dark-field ophthalmic contact probe for analysis of aqueous humor (AH) was designed by ray tracing and constructed in quartz glass for experimental use as a prototype probe in a confocal Raman spectroscopic setup. The misalignment tolerance of this probe was theoretically evaluated, whereas the optical performance was experimentally evaluated by a concentration curve of the ocular drug phenylephrine HCl in an artificial eye model. The results indicated a ninefold improvement in misalignment tolerance and a 36-fold improvement in the signal-to-noise ratio at the phenylephrine HCl 1002 cm(-1) peak compared with a previous design. The prototype showed a good linear fit with the phenylephrine HCl concentration, with an R-2 of 0.996. Phenylephrine HCl concentration down to 0.1% is detectable.",

keywords = "aqueous humor, dark&#8208, field illumination, ocular drug, Raman spectroscopy",

author = "Shuo Zhang and Erckens, {Roel J.} and Jongsma, {Franciscus H. M.} and Webers, {Carroll A. B.} and Berendschot, {Tos T. J. M.}",

note = "Funding Information: The first author was supported by the China Scholarship Council under State Scholarship Fund No. 201309110103. Publisher Copyright: {\textcopyright} 2021 The Authors. Journal of Raman Spectroscopy published by John Wiley & Sons Ltd.",

year = "2021",

month = jul,

doi = "10.1002/jrs.6125",

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T1 - Design and performance of a dark-field probe with confocal Raman spectroscopy for ophthalmic applications

AU - Zhang, Shuo

AU - Erckens, Roel J.

AU - Jongsma, Franciscus H. M.

AU - Webers, Carroll A. B.

AU - Berendschot, Tos T. J. M.

N1 - Funding Information: The first author was supported by the China Scholarship Council under State Scholarship Fund No. 201309110103. Publisher Copyright: © 2021 The Authors. Journal of Raman Spectroscopy published by John Wiley & Sons Ltd.

PY - 2021/7

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N2 - A dark-field ophthalmic contact probe for analysis of aqueous humor (AH) was designed by ray tracing and constructed in quartz glass for experimental use as a prototype probe in a confocal Raman spectroscopic setup. The misalignment tolerance of this probe was theoretically evaluated, whereas the optical performance was experimentally evaluated by a concentration curve of the ocular drug phenylephrine HCl in an artificial eye model. The results indicated a ninefold improvement in misalignment tolerance and a 36-fold improvement in the signal-to-noise ratio at the phenylephrine HCl 1002 cm(-1) peak compared with a previous design. The prototype showed a good linear fit with the phenylephrine HCl concentration, with an R-2 of 0.996. Phenylephrine HCl concentration down to 0.1% is detectable.

AB - A dark-field ophthalmic contact probe for analysis of aqueous humor (AH) was designed by ray tracing and constructed in quartz glass for experimental use as a prototype probe in a confocal Raman spectroscopic setup. The misalignment tolerance of this probe was theoretically evaluated, whereas the optical performance was experimentally evaluated by a concentration curve of the ocular drug phenylephrine HCl in an artificial eye model. The results indicated a ninefold improvement in misalignment tolerance and a 36-fold improvement in the signal-to-noise ratio at the phenylephrine HCl 1002 cm(-1) peak compared with a previous design. The prototype showed a good linear fit with the phenylephrine HCl concentration, with an R-2 of 0.996. Phenylephrine HCl concentration down to 0.1% is detectable.

KW - aqueous humor

KW - dark&#8208

KW - field illumination

KW - ocular drug

KW - Raman spectroscopy

U2 - 10.1002/jrs.6125

DO - 10.1002/jrs.6125

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