Delivery of DNA into the central nervous system via electroporation

Research output: Chapter in Book/Report/Conference proceedingChapterAcademic

2 Citations (Scopus)

Abstract

Electroporation of non-viral plasmid DNA is a valuable tool to alter gene expression in the adult central nervous system. It offers a number of advantages over viral gene delivery as non-viral plasmids can integrate larger inserts and reduce the risk of inducing unintended immunological responses. Generally, electroporation of the adult brain is accomplished in rodents by applying high-amplitude voltage-controlled pulses through the entire brain with plate electrodes surrounding the animal's head. Here, we describe an alternative electroporation protocol making use of current-controlled low-amplitude pulses that are delivered locally by means of needlelike electrodes in the brain of adult mice. This allows altering gene expression in very-well-defined areas of the brain while inducing minimal tissue damage.

Original languageEnglish
Title of host publicationMethods in molecular biology
Pages157-63
Number of pages7
Volume1121
DOIs
Publication statusPublished - 2014

Keywords

  • Analgesia
  • Animals
  • Central Nervous System
  • DNA
  • Electroporation
  • Injections
  • Mice
  • Mice, Inbred C57BL
  • Stereotaxic Techniques
  • Transfection

Cite this

@inbook{cade2cc9b3c348319ebdb1770d36c60d,
title = "Delivery of DNA into the central nervous system via electroporation",
abstract = "Electroporation of non-viral plasmid DNA is a valuable tool to alter gene expression in the adult central nervous system. It offers a number of advantages over viral gene delivery as non-viral plasmids can integrate larger inserts and reduce the risk of inducing unintended immunological responses. Generally, electroporation of the adult brain is accomplished in rodents by applying high-amplitude voltage-controlled pulses through the entire brain with plate electrodes surrounding the animal's head. Here, we describe an alternative electroporation protocol making use of current-controlled low-amplitude pulses that are delivered locally by means of needlelike electrodes in the brain of adult mice. This allows altering gene expression in very-well-defined areas of the brain while inducing minimal tissue damage.",
keywords = "Analgesia, Animals, Central Nervous System, DNA, Electroporation, Injections, Mice, Mice, Inbred C57BL, Stereotaxic Techniques, Transfection",
author = "{De Vry}, Jochen and Tim Vanmierlo and Pilar Mart{\'i}nez-Mart{\'i}nez and Mario Losen and Yasin Temel and Harry Steinbusch and {De Baets}, Marc and Jos Prickaerts",
year = "2014",
doi = "10.1007/978-1-4614-9632-8_14",
language = "English",
volume = "1121",
pages = "157--63",
booktitle = "Methods in molecular biology",

}

Delivery of DNA into the central nervous system via electroporation. / De Vry, Jochen; Vanmierlo, Tim; Martínez-Martínez, Pilar; Losen, Mario; Temel, Yasin; Steinbusch, Harry; De Baets, Marc; Prickaerts, Jos.

Methods in molecular biology. Vol. 1121 2014. p. 157-63.

Research output: Chapter in Book/Report/Conference proceedingChapterAcademic

TY - CHAP

T1 - Delivery of DNA into the central nervous system via electroporation

AU - De Vry, Jochen

AU - Vanmierlo, Tim

AU - Martínez-Martínez, Pilar

AU - Losen, Mario

AU - Temel, Yasin

AU - Steinbusch, Harry

AU - De Baets, Marc

AU - Prickaerts, Jos

PY - 2014

Y1 - 2014

N2 - Electroporation of non-viral plasmid DNA is a valuable tool to alter gene expression in the adult central nervous system. It offers a number of advantages over viral gene delivery as non-viral plasmids can integrate larger inserts and reduce the risk of inducing unintended immunological responses. Generally, electroporation of the adult brain is accomplished in rodents by applying high-amplitude voltage-controlled pulses through the entire brain with plate electrodes surrounding the animal's head. Here, we describe an alternative electroporation protocol making use of current-controlled low-amplitude pulses that are delivered locally by means of needlelike electrodes in the brain of adult mice. This allows altering gene expression in very-well-defined areas of the brain while inducing minimal tissue damage.

AB - Electroporation of non-viral plasmid DNA is a valuable tool to alter gene expression in the adult central nervous system. It offers a number of advantages over viral gene delivery as non-viral plasmids can integrate larger inserts and reduce the risk of inducing unintended immunological responses. Generally, electroporation of the adult brain is accomplished in rodents by applying high-amplitude voltage-controlled pulses through the entire brain with plate electrodes surrounding the animal's head. Here, we describe an alternative electroporation protocol making use of current-controlled low-amplitude pulses that are delivered locally by means of needlelike electrodes in the brain of adult mice. This allows altering gene expression in very-well-defined areas of the brain while inducing minimal tissue damage.

KW - Analgesia

KW - Animals

KW - Central Nervous System

KW - DNA

KW - Electroporation

KW - Injections

KW - Mice

KW - Mice, Inbred C57BL

KW - Stereotaxic Techniques

KW - Transfection

U2 - 10.1007/978-1-4614-9632-8_14

DO - 10.1007/978-1-4614-9632-8_14

M3 - Chapter

C2 - 24510821

VL - 1121

SP - 157

EP - 163

BT - Methods in molecular biology

ER -