Deciphering Metabolic Heterogeneity by Single-Cell Analysis

Tom M. J. Evers; Mazene Hochane; Sander J. Tans; Ron M. A. Heeren; Stefan Semrau; Peter Nemes; Alireza Mashaghi

doi:10.1021/acs.analchem.9b02410

Deciphering Metabolic Heterogeneity by Single-Cell Analysis

Tom M. J. Evers, Mazene Hochane, Sander J. Tans, Ron M. A. Heeren, Stefan Semrau, Peter Nemes, Alireza Mashaghi^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

44 Citations (Web of Science)

Abstract

Single-cell analysis provides insights into cellular heterogeneity and dynamics of individual cells. This Feature highlights recent developments in key analytical techniques suited for single-cell metabolic analysis with a special focus on mass spectrometry-based analytical platforms and RNA-seq as well as imaging techniques that reveal stochasticity in metabolism.

Original language	English
Pages (from-to)	13314-13323
Number of pages	10
Journal	Analytical Chemistry
Volume	91
Issue number	21
DOIs	https://doi.org/10.1021/acs.analchem.9b02410
Publication status	Published - 5 Nov 2019

Keywords

IONIZATION MASS-SPECTROMETRY
CAPILLARY-ELECTROPHORESIS
RNA-SEQ
EMBRYONIC-CELLS
LIVE CELLS
DYNAMICS
REVEALS
PROTEOMICS
CYTOMETRY
PROTEIN

Access to Document

10.1021/acs.analchem.9b02410

Cite this

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title = "Deciphering Metabolic Heterogeneity by Single-Cell Analysis",

abstract = "Single-cell analysis provides insights into cellular heterogeneity and dynamics of individual cells. This Feature highlights recent developments in key analytical techniques suited for single-cell metabolic analysis with a special focus on mass spectrometry-based analytical platforms and RNA-seq as well as imaging techniques that reveal stochasticity in metabolism.",

keywords = "IONIZATION MASS-SPECTROMETRY, CAPILLARY-ELECTROPHORESIS, RNA-SEQ, EMBRYONIC-CELLS, LIVE CELLS, DYNAMICS, REVEALS, PROTEOMICS, CYTOMETRY, PROTEIN",

author = "Evers, {Tom M. J.} and Mazene Hochane and Tans, {Sander J.} and Heeren, {Ron M. A.} and Stefan Semrau and Peter Nemes and Alireza Mashaghi",

year = "2019",

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day = "5",

doi = "10.1021/acs.analchem.9b02410",

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T1 - Deciphering Metabolic Heterogeneity by Single-Cell Analysis

AU - Evers, Tom M. J.

AU - Hochane, Mazene

AU - Tans, Sander J.

AU - Heeren, Ron M. A.

AU - Semrau, Stefan

AU - Nemes, Peter

AU - Mashaghi, Alireza

PY - 2019/11/5

Y1 - 2019/11/5

N2 - Single-cell analysis provides insights into cellular heterogeneity and dynamics of individual cells. This Feature highlights recent developments in key analytical techniques suited for single-cell metabolic analysis with a special focus on mass spectrometry-based analytical platforms and RNA-seq as well as imaging techniques that reveal stochasticity in metabolism.

AB - Single-cell analysis provides insights into cellular heterogeneity and dynamics of individual cells. This Feature highlights recent developments in key analytical techniques suited for single-cell metabolic analysis with a special focus on mass spectrometry-based analytical platforms and RNA-seq as well as imaging techniques that reveal stochasticity in metabolism.

KW - IONIZATION MASS-SPECTROMETRY

KW - CAPILLARY-ELECTROPHORESIS

KW - RNA-SEQ

KW - EMBRYONIC-CELLS

KW - LIVE CELLS

KW - DYNAMICS

KW - REVEALS

KW - PROTEOMICS

KW - CYTOMETRY

KW - PROTEIN

U2 - 10.1021/acs.analchem.9b02410

DO - 10.1021/acs.analchem.9b02410

M3 - Article

VL - 91

SP - 13314

EP - 13323

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 21

ER -