Decellularization of porcine heart tissue to obtain extracellular matrix based hydrogels

Rabeil Sakina; Aida Llucià-Valldeperas; Ana Henriques Lourenço; Abhishek Harichandan; Sandro Gelsomino; Paul Wieringa; Carlos Mota; Lorenzo Moroni

doi:10.1016/bs.mcb.2019.11.023

Decellularization of porcine heart tissue to obtain extracellular matrix based hydrogels

Rabeil Sakina, Aida Llucià-Valldeperas, Ana Henriques Lourenço, Abhishek Harichandan, Sandro Gelsomino, Paul Wieringa, Carlos Mota, Lorenzo Moroni^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic

190 Downloads (Pure)

Abstract

The use of hydrogels derived from the extracellular matrix (ECM) in tissue engineering applications aims to overcome the conundrum of mimicking the complexity of ECM composition in vitro. In this chapter, we describe a method of decellularization and subsequent formation of an ECM-based hydrogel using porcine heart tissue. These decellularized ECM hydrogels could be used to create semi-interpenetrating networks or as bioinks in bioprinting applications to further enhance the bioactivity and increase the biomimicry degree of the biological cardiac constructs.

Original language	English
Title of host publication	Cell-derived Matrices - Part B
Editors	David Caballero, Subhas C. Kundu, Rui L. Reis
Publisher	Elsevier Inc.
Chapter	1
Pages	3-21
Number of pages	19
Volume	157
ISBN (Print)	978-0-12-820174-9
DOIs	https://doi.org/10.1016/bs.mcb.2019.11.023
Publication status	Published - 2020

Publication series

Series	Methods in Cell Biology
ISSN	0091-679X

Keywords

Cardiac
Decellularized
Extracellular matrix
Hydrogel
Tissue engineering
BIOLOGIC SCAFFOLDS
MYOCARDIAL-INFARCTION
REGENERATION
CHITOSAN
FAILURE
PEPTIDE
CELL-CULTURE
DIFFERENTIATION
SKELETAL

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10.1016/bs.mcb.2019.11.023

Full text Final published version, 2.51 MBLicence: Taverne

Cite this

Sakina, R., Llucià-Valldeperas, A., Henriques Lourenço, A., Harichandan, A., Gelsomino, S., Wieringa, P., Mota, C., & Moroni, L. (2020). Decellularization of porcine heart tissue to obtain extracellular matrix based hydrogels. In D. Caballero, S. C. Kundu, & R. L. Reis (Eds.), Cell-derived Matrices - Part B (Vol. 157, pp. 3-21). Elsevier Inc.. https://doi.org/10.1016/bs.mcb.2019.11.023

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abstract = "The use of hydrogels derived from the extracellular matrix (ECM) in tissue engineering applications aims to overcome the conundrum of mimicking the complexity of ECM composition in vitro. In this chapter, we describe a method of decellularization and subsequent formation of an ECM-based hydrogel using porcine heart tissue. These decellularized ECM hydrogels could be used to create semi-interpenetrating networks or as bioinks in bioprinting applications to further enhance the bioactivity and increase the biomimicry degree of the biological cardiac constructs.",

keywords = "Cardiac, Decellularized, Extracellular matrix, Hydrogel, Tissue engineering, BIOLOGIC SCAFFOLDS, MYOCARDIAL-INFARCTION, REGENERATION, CHITOSAN, FAILURE, PEPTIDE, CELL-CULTURE, DIFFERENTIATION, SKELETAL",

author = "Rabeil Sakina and Aida Lluci{\`a}-Valldeperas and {Henriques Louren{\c c}o}, Ana and Abhishek Harichandan and Sandro Gelsomino and Paul Wieringa and Carlos Mota and Lorenzo Moroni",

year = "2020",

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volume = "157",

series = "Methods in Cell Biology",

publisher = "Elsevier Inc.",

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Sakina, R, Llucià-Valldeperas, A, Henriques Lourenço, A, Harichandan, A, Gelsomino, S , Wieringa, P , Mota, C & Moroni, L 2020, Decellularization of porcine heart tissue to obtain extracellular matrix based hydrogels. in D Caballero, SC Kundu & RL Reis (eds), Cell-derived Matrices - Part B. vol. 157, Elsevier Inc., Methods in Cell Biology, pp. 3-21. https://doi.org/10.1016/bs.mcb.2019.11.023

Decellularization of porcine heart tissue to obtain extracellular matrix based hydrogels. / Sakina, Rabeil; Llucià-Valldeperas, Aida; Henriques Lourenço, Ana et al.
Cell-derived Matrices - Part B. ed. / David Caballero; Subhas C. Kundu; Rui L. Reis. Vol. 157 Elsevier Inc., 2020. p. 3-21 (Methods in Cell Biology).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic

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T1 - Decellularization of porcine heart tissue to obtain extracellular matrix based hydrogels

AU - Sakina, Rabeil

AU - Llucià-Valldeperas, Aida

AU - Henriques Lourenço, Ana

AU - Harichandan, Abhishek

AU - Gelsomino, Sandro

AU - Wieringa, Paul

AU - Mota, Carlos

AU - Moroni, Lorenzo

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N2 - The use of hydrogels derived from the extracellular matrix (ECM) in tissue engineering applications aims to overcome the conundrum of mimicking the complexity of ECM composition in vitro. In this chapter, we describe a method of decellularization and subsequent formation of an ECM-based hydrogel using porcine heart tissue. These decellularized ECM hydrogels could be used to create semi-interpenetrating networks or as bioinks in bioprinting applications to further enhance the bioactivity and increase the biomimicry degree of the biological cardiac constructs.

AB - The use of hydrogels derived from the extracellular matrix (ECM) in tissue engineering applications aims to overcome the conundrum of mimicking the complexity of ECM composition in vitro. In this chapter, we describe a method of decellularization and subsequent formation of an ECM-based hydrogel using porcine heart tissue. These decellularized ECM hydrogels could be used to create semi-interpenetrating networks or as bioinks in bioprinting applications to further enhance the bioactivity and increase the biomimicry degree of the biological cardiac constructs.

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KW - Extracellular matrix

KW - Hydrogel

KW - Tissue engineering

KW - BIOLOGIC SCAFFOLDS

KW - MYOCARDIAL-INFARCTION

KW - REGENERATION

KW - CHITOSAN

KW - FAILURE

KW - PEPTIDE

KW - CELL-CULTURE

KW - DIFFERENTIATION

KW - SKELETAL

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VL - 157

T3 - Methods in Cell Biology

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