Data management in Thrombin Generation

H. Coenraad Hemker*, R. Kremers

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

83 Citations (Web of Science)

Abstract

To obtain a thrombin generation (TG) curve from the conversion of added fluorogenic substrate, thrombin concentrations are to be derived from the observed velocity of increase of fluorescence (dF/dt). The relation between velocity and thrombin concentration varies during the experiment because substrate is consumed and because fluorescence is not linear with the concentration of product. Here we review the techniques that we developed to: A: Transform the fluorescence trace into the "ideal" trace that would be seen if substrate consumption and non-linearity of fluorescence would not play a role. B: Subtract the contribution of alpha M-2-thrombin so as to obtain the course of free thrombin. C: Calculate the velocity of prothrombin conversion from the course of free thrombin. D: Fit a smooth curve through the points obtained in a TG experiment
Original languageEnglish
Pages (from-to)3-11
JournalThrombosis Research
Volume131
Issue number1
DOIs
Publication statusPublished - Jan 2013

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