Dark-field illumination in conjunction with confocal Raman spectroscopy for real-time noninvasive aqueous humor investigation

Shuo Zhang; Roel J. Erckens; Frans H. M. Jongsma; John De Brabander; Carroll A. B. Webers; Tos T. J. M. Berendschot

doi:10.1117/1.OE.59.9.092002

Dark-field illumination in conjunction with confocal Raman spectroscopy for real-time noninvasive aqueous humor investigation

Shuo Zhang, Roel J. Erckens, Frans H. M. Jongsma, John De Brabander, Carroll A. B. Webers, Tos T. J. M. Berendschot^*

^*Corresponding author for this work

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Abstract

To obtain a real-time noninvasive analysis of the content of the aqueous humor (AH) in the eye, a confocal dark-field ophthalmic probe was designed and optimized by ray-tracing software. The confocal technique allows Raman spectrometric sampling of the AH with suppressing signals from the surrounding tissues. Dark-field illumination prevents the excitation light to reach the vulnerable retinal tissue directly. In order to evaluate the optical performance and safety of this method, a functional prototyped eye contact probe has been designed and tested on ex vivo rabbit eyes. (C) 2020 Society of Photo-Optical Instrumentation Engineers (SPIE)

Original language	English
Article number	092002
Number of pages	9
Journal	Optical Engineering
Volume	59
Issue number	9
DOIs	https://doi.org/10.1117/1.OE.59.9.092002
Publication status	Published - Sep 2020

Keywords

Raman spectroscopy
eye
aqueous humor
confocal microscopy
dark-field illumination
TISSUES
GLUCOSE

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10.1117/1.OE.59.9.092002

Full TextFinal published version, 1.04 MBLicence: Taverne

Cite this

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title = "Dark-field illumination in conjunction with confocal Raman spectroscopy for real-time noninvasive aqueous humor investigation",

abstract = "To obtain a real-time noninvasive analysis of the content of the aqueous humor (AH) in the eye, a confocal dark-field ophthalmic probe was designed and optimized by ray-tracing software. The confocal technique allows Raman spectrometric sampling of the AH with suppressing signals from the surrounding tissues. Dark-field illumination prevents the excitation light to reach the vulnerable retinal tissue directly. In order to evaluate the optical performance and safety of this method, a functional prototyped eye contact probe has been designed and tested on ex vivo rabbit eyes. (C) 2020 Society of Photo-Optical Instrumentation Engineers (SPIE)",

keywords = "Raman spectroscopy, eye, aqueous humor, confocal microscopy, dark-field illumination, TISSUES, GLUCOSE",

author = "Shuo Zhang and Erckens, {Roel J.} and Jongsma, {Frans H. M.} and {De Brabander}, John and Webers, {Carroll A. B.} and Berendschot, {Tos T. J. M.}",

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AU - Zhang, Shuo

AU - Erckens, Roel J.

AU - Jongsma, Frans H. M.

AU - De Brabander, John

AU - Webers, Carroll A. B.

AU - Berendschot, Tos T. J. M.

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AB - To obtain a real-time noninvasive analysis of the content of the aqueous humor (AH) in the eye, a confocal dark-field ophthalmic probe was designed and optimized by ray-tracing software. The confocal technique allows Raman spectrometric sampling of the AH with suppressing signals from the surrounding tissues. Dark-field illumination prevents the excitation light to reach the vulnerable retinal tissue directly. In order to evaluate the optical performance and safety of this method, a functional prototyped eye contact probe has been designed and tested on ex vivo rabbit eyes. (C) 2020 Society of Photo-Optical Instrumentation Engineers (SPIE)

KW - Raman spectroscopy

KW - eye

KW - aqueous humor

KW - confocal microscopy

KW - dark-field illumination

KW - TISSUES

KW - GLUCOSE

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JO - Optical Engineering

JF - Optical Engineering

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