Creeping Proteins in Microporous Structures: Polymer Brush-Assisted Fabrication of 3D Gradients for Tissue Engineering

Michel Klein Gunnewiek, Andrea Di Luca, Hermannes Z. Bollemaat, Clemens A. van Blitterswijk, G. Julius Vancso, Lorenzo Moroni*, Edmondo M. Benetti

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Coupling of rapid prototyping techniques and surface-confined polymerizations allows the fabrication of 3D multidirectional gradients of biomolecules within microporous scaffolds. The compositional gradients can be tailored by polymer-brush-assisted diffusion of protein solutions. This technique allows spatial control over stem cells manipulation within 3D environments. ? 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Original languageEnglish
Pages (from-to)1169-1174
JournalAdvanced Healthcare Materials
Issue number8
Publication statusPublished - 3 Jun 2015


  • 3D scaffolds
  • poly(epsilon-caprolactone)
  • polymer brushes
  • protein gradients
  • wetting

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