TY - JOUR
T1 - Coordinated regulation of skeletal muscle mass and metabolic plasticity during recovery from disuse
AU - Kneppers, Anita
AU - Leermakers, Pieter
AU - Pansters, Nicholas
AU - Backx, Evelien
AU - Gosker, Harry
AU - van Loon, Luc
AU - Schols, Annemie
AU - Langen, Ramon
AU - Verdijk, Lex
N1 - Funding Information:
The authors thank Marco Kelders, Chiel de Theije, and Antoine Zorenc (all of Maastricht University) for excellent technical assistance in performing the molecular analyses, and Roy Haast (Maastricht Centre for Systems Biology, Maastricht University) for support in conducting the cluster analysis. %blankline%
Funding Information:
The authors thank Marco Kelders, Chiel de Theije, and Antoine Zorenc (all of Maastricht University) for excellent technical assistance in performing the molecular analyses, and Roy Haast (Maastricht Centre for Systems Biology, Maastricht University) for support in conducting the cluster analysis. The original work from which human muscle biopsies were obtained (36) was funded by TI Food and Nutrition, a public–private partnership for precompetitive research in food and nutrition; and the mouse study was funded by Grant NAF 3.2.07.017 from the Lung Foundation/Netherlands Asthma Foundation and the transnational University Limburg (tUL). For the current work, the researchers had sole responsibility for the study design, data collection and analysis, decision to publish, and preparation of the manuscript. The authors declare no conflicts of interest.
Publisher Copyright:
© FASEB.
PY - 2019/1
Y1 - 2019/1
N2 - Skeletal muscle regeneration after disuse is essential for muscle maintenance and involves the regulation of both mass- and metabolic plasticity-related processes. However, the relation between these processes during recovery from disuse remains unclear. In this study, we explored the potential interrelationship between the molecular regulation of muscle mass and oxidative metabolism during recovery from disuse. Molecular profiles were measured in biopsies from the vastus lateralis of healthy men after 1-leg cast immobilization and after 1 wk reloading, and in mouse gastrocnemius obtained before and after hindlimb suspension and during reloading (RL-1, -2, -3, -5, and -8 d). Cluster analysis of the human recovery response revealed correlations between myogenesis and autophagy markers in 2 clusters, which were distinguished by the presence of markers of early myogenesis, autophagosome formation, and mitochondrial turnover vs. markers of late myogenesis, autophagy initiation, and mitochondrial mass. In line with these findings, an early transient increase in B-cell lymphoma-2 interacting protein-3 and sequestosome-1 protein, and GABA type A receptor-associated protein like-1 protein and mRNA and a late increase in myomaker and myosin heavy chain-8 mRNA, microtubule-associated protein 1 light chain 3-II:I ratio, and FUN14 domain-containing-1 mRNA and protein were observed in mice. In summary, the regulatory profiles of protein, mitochondrial, and myonuclear turnover are correlated and temporally associated, suggesting a coordinated regulation of muscle mass- and oxidative metabolism-related processes during recovery from disuse.Kneppers, A., Leermakers, P., Pansters, N., Backx, E., Gosker, H., van Loon, L., Schols, A., Langen, R., Verdijk, L. Coordinated regulation of skeletal muscle mass and metabolic plasticity during recovery from disuse.
AB - Skeletal muscle regeneration after disuse is essential for muscle maintenance and involves the regulation of both mass- and metabolic plasticity-related processes. However, the relation between these processes during recovery from disuse remains unclear. In this study, we explored the potential interrelationship between the molecular regulation of muscle mass and oxidative metabolism during recovery from disuse. Molecular profiles were measured in biopsies from the vastus lateralis of healthy men after 1-leg cast immobilization and after 1 wk reloading, and in mouse gastrocnemius obtained before and after hindlimb suspension and during reloading (RL-1, -2, -3, -5, and -8 d). Cluster analysis of the human recovery response revealed correlations between myogenesis and autophagy markers in 2 clusters, which were distinguished by the presence of markers of early myogenesis, autophagosome formation, and mitochondrial turnover vs. markers of late myogenesis, autophagy initiation, and mitochondrial mass. In line with these findings, an early transient increase in B-cell lymphoma-2 interacting protein-3 and sequestosome-1 protein, and GABA type A receptor-associated protein like-1 protein and mRNA and a late increase in myomaker and myosin heavy chain-8 mRNA, microtubule-associated protein 1 light chain 3-II:I ratio, and FUN14 domain-containing-1 mRNA and protein were observed in mice. In summary, the regulatory profiles of protein, mitochondrial, and myonuclear turnover are correlated and temporally associated, suggesting a coordinated regulation of muscle mass- and oxidative metabolism-related processes during recovery from disuse.Kneppers, A., Leermakers, P., Pansters, N., Backx, E., Gosker, H., van Loon, L., Schols, A., Langen, R., Verdijk, L. Coordinated regulation of skeletal muscle mass and metabolic plasticity during recovery from disuse.
KW - remobilization
KW - remodeling
KW - protein turnover
KW - myogenesis
KW - mitophagy
KW - SATELLITE CELL
KW - MITOCHONDRIAL DYNAMICS
KW - PGC-1-ALPHA OVEREXPRESSION
KW - CREATINE SUPPLEMENTATION
KW - LEG IMMOBILIZATION
KW - GENE-EXPRESSION
KW - FIBER SIZE
KW - ATROPHY
KW - REGENERATION
KW - EXERCISE
U2 - 10.1096/fj.201701403rrr
DO - 10.1096/fj.201701403rrr
M3 - Article
C2 - 30133324
SN - 0892-6638
VL - 33
SP - 1288
EP - 1298
JO - Faseb Journal
JF - Faseb Journal
IS - 1
ER -