Controlled aggregation of primary human pancreatic islet cells leads to glucose-responsive pseudoislets comparable to native islets

Janneke Hilderink, Siebe Spijker, Françoise Carlotti, Lydia Lange, Marten Engelse, Clemens van Blitterswijk, Eelco de Koning, Marcel Karperien, Aart van Apeldoorn

Research output: Chapter in Book/Report/Conference proceedingChapterAcademic

Abstract

Clinical islet transplantation is a promising treatment for patients with type 1 diabetes. However, pancreatic islets vary in size and shape affecting their survival and function after transplantation because of mass transport limitations. To reduce diffusion restrictions and improve islet cell survival, the generation of islets with optimal dimensions by dispersion followed by reassembly of islet cells, can help limit the length of diffusion pathways. This study describes a microwell platform that supports the controlled and reproducible production of three-dimensional pancreatic cell clusters of human donor islets. We observed that primary human islet cell aggregates with a diameter of 100-150 μm consisting of about 1000 cells best resembled intact pancreatic islets as they showed low apoptotic cell death (
Original languageEnglish
Title of host publicationJournal of cellular and molecular medicine
Pages1836-46
Number of pages11
DOIs
Publication statusPublished - 2015

Publication series

SeriesJournal of cellular and molecular medicine
Volume19

Keywords

  • islets type 1 diabetes
  • pseudoislets bioengineering beta cells

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