Controlled aggregation of primary human pancreatic islet cells leads to glucose-responsive pseudoislets comparable to native islets

Janneke Hilderink; Siebe Spijker; Françoise Carlotti; Lydia Lange; Marten Engelse; Clemens van Blitterswijk; Eelco de Koning; Marcel Karperien; Aart van Apeldoorn

doi:10.1111/jcmm.12555

Controlled aggregation of primary human pancreatic islet cells leads to glucose-responsive pseudoislets comparable to native islets

Janneke Hilderink, Siebe Spijker, Françoise Carlotti, Lydia Lange, Marten Engelse, Clemens van Blitterswijk, Eelco de Koning, Marcel Karperien, Aart van Apeldoorn^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic

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Abstract

Clinical islet transplantation is a promising treatment for patients with type 1 diabetes. However, pancreatic islets vary in size and shape affecting their survival and function after transplantation because of mass transport limitations. To reduce diffusion restrictions and improve islet cell survival, the generation of islets with optimal dimensions by dispersion followed by reassembly of islet cells, can help limit the length of diffusion pathways. This study describes a microwell platform that supports the controlled and reproducible production of three-dimensional pancreatic cell clusters of human donor islets. We observed that primary human islet cell aggregates with a diameter of 100-150 μm consisting of about 1000 cells best resembled intact pancreatic islets as they showed low apoptotic cell death (

Original language	English
Title of host publication	Journal of cellular and molecular medicine
Pages	1836-46
Number of pages	11
DOIs	https://doi.org/10.1111/jcmm.12555
Publication status	Published - 2015

Publication series

Series	Journal of cellular and molecular medicine
Volume	19

Keywords

islets type 1 diabetes
pseudoislets bioengineering beta cells

Access to Document

10.1111/jcmm.12555Licence: CC BY

TaverneFinal published version, 1.06 MBLicence: Taverne

Cite this

Hilderink, J., Spijker, S., Carlotti, F., Lange, L., Engelse, M., van Blitterswijk, C., de Koning, E., Karperien, M., & van Apeldoorn, A. (2015). Controlled aggregation of primary human pancreatic islet cells leads to glucose-responsive pseudoislets comparable to native islets. In Journal of cellular and molecular medicine (pp. 1836-46) https://doi.org/10.1111/jcmm.12555

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Hilderink, J, Spijker, S, Carlotti, F, Lange, L, Engelse, M, van Blitterswijk, C, de Koning, E, Karperien, M & van Apeldoorn, A 2015, Controlled aggregation of primary human pancreatic islet cells leads to glucose-responsive pseudoislets comparable to native islets. in Journal of cellular and molecular medicine. Journal of cellular and molecular medicine, vol. 19, pp. 1836-46. https://doi.org/10.1111/jcmm.12555

Controlled aggregation of primary human pancreatic islet cells leads to glucose-responsive pseudoislets comparable to native islets. / Hilderink, Janneke; Spijker, Siebe; Carlotti, Françoise et al.
Journal of cellular and molecular medicine. 2015. p. 1836-46 (Journal of cellular and molecular medicine, Vol. 19).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic

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AU - Engelse, Marten

AU - van Blitterswijk, Clemens

AU - de Koning, Eelco

AU - Karperien, Marcel

AU - van Apeldoorn, Aart

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