Consistent expression pattern of myogenic regulatory factors in whole muscle and isolated human muscle satellite cells after eccentric contractions in humans

Joshua P. Nederveen, Stephen A. Fortino, Jeff M. Baker, Tim Snijders, Sophie Joanisse, Chris McGlory, Bryon R. McKay, Dinesh Kumbhare, Gianni Parise*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Web of Science)

Abstract

Skeletal muscle satellite cells (SC) play an important role in muscle repair following injury. The regulation of SC activity is governed by myogenic regulatory factors (MRF), including MyoD, Myf5, myogenin, and MRF4. The mRNA expression of these MRF in humans following muscle damage has been predominately measured in whole muscle homogenates. Whether the temporal expression of MRF in a whole muscle homogenate reflects SC-specific expression of MRF remains largely unknown. Sixteen young men (23.1 +/- 1.0 yr) performed 300 unilateral eccentric contractions (180 degrees/s) of the knee extensors. Percutaneous muscle biopsies from the vastus lateralis were taken before (Pre) and 48 h postexercise. Fluorescence-activated cell sorting analysis was utilized to purify NCAM + muscle SC from the whole muscle homogenate. Forty-eight hours post-eccentric exercise. MyoD, Myf5, and myogenin mRNA expression were increased in the whole muscle homogenate (similar to 1.4-, similar to 4.0-, similar to 1.7-fold, respectively, P <0.05) and in isolated SC (similar to 19.3-, similar to 17.5-, similar to 58.9-fold, respectively, P <0.05). MRF4 mRNA expression was not increased 48 h postexercise in the whole muscle homogenate (P > 0.05) or in isolated SC (P > 0.05). In conclusion, our results suggest that the directional changes in mRNA expression of the MRF in a whole muscle homogenate in response to acute eccentric exercise reflects that observed in isolated muscle SC.

NEW & NOTEWORTHY The myogenic program is controlled via transcription factors referred to as myogenic regulatory factors (MRF). Previous studies have derived MRF expression from whole muscle homogenates, but little work has examined whether the mRNA expression of these transcripts reflects the pattern of expression in the actual population of satellite cells (SC). We report that MRF expression from an enriched SC population reflects the directional pattern of expression from skeletal muscle biopsy samples following eccentric contractions.

Original languageEnglish
Pages (from-to)1419-1426
Number of pages8
JournalJournal of Applied Physiology
Volume127
Issue number5
DOIs
Publication statusPublished - Nov 2019

Keywords

  • fluorescence-activated cell sorting
  • mRNA expression
  • myogenic regulator factors
  • satellite cell
  • HUMAN SKELETAL-MUSCLE
  • MESSENGER-RNA MIGHT
  • EXERCISE ADAPTATIONS
  • LENGTHENING CONTRACTIONS
  • STEM-CELLS
  • YOUNG
  • ACTIVATION
  • DAMAGE
  • MYOD
  • OPTIMIZATION

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