Consensus guidelines for the use and interpretation of angiogenesis assays

Patrycja Nowak-Sliwinska*, Kari Alitalo, Elizabeth Allen, Andrey Anisimov, Alfred C. Aplin, Robert Auerbach, Hellmut G. Augustin, David O. Bates, Judy R. van Beijnum, R. Hugh F. Bender, Gabriele Bergers, Andreas Bikfalvi, Joyce Bischoff, Barbara C. Bock, Peter C. Brooks, Federico Bussolino, Bertan Cakir, Peter Carmeliet, Daniel Castranova, Anca M. CimpeanOndine Cleaver, George Coukos, George E. Davis, Michele De Palma, Anna Dimberg, Ruud P. M. Dings, Valentin Djonov, Andrew C. Dudley, Neil P. Dufton, Sarah-Maria Fendt, Napoleone Ferrara, Marcus Fruttiger, Dai Fukumura, Bart Ghesquiere, Yan Gong, Robert J. Griffin, Adrian L. Harris, Christopher C. W. Hughes, Nan W. Hultgren, M. Luisa Iruela-Arispe, Melita Irving, Rakesh K. Jain, Raghu Kalluri, Joanna Kalucka, Robert S. Kerbel, Jan Kitajewski, Ingeborg Klaassen, Hynda K. Kleinmann, Pieter Koolwijk, Elisabeth Kuczynski, Brenda R. Kwak, Koen Marien, Juan M. Melero-Martin, Lance L. Munn, Roberto F. Nicosia, Agnes Noel, Jussi Nurro, Anna-Karin Olsson, Tatiana V. Petrova, Kristian Pietras, Roberto Pili, Jeffrey W. Pollard, Mark J. Post, Paul H. A. Quax, Gabriel A. Rabinovich, Marius Raica, Anna M. Randi, Domenico Ribatti, Curzio Ruegg, Reinier O. Schlingemann, Stefan Schulte-Merker, Lois E. H. Smith, Jonathan W. Song, Steven A. Stacker, Jimmy Stalin, Amber N. Stratman, Maureen Van de Velde, Victor W. M. van Hinsbergh, Peter B. Vermeulen, Johannes Waltenberger, Brant M. Weinstein, Hong Xin, Bahar Yetkin-Arik, Seppo Yla-Herttuala, Mervin C. Yoder, Arjan W. Griffioen*

*Corresponding author for this work

Research output: Contribution to journal(Systematic) Review article peer-review

295 Citations (Web of Science)

Abstract

The formation of new blood vessels, or angiogenesis, is a complex process that plays important roles in growth and development, tissue and organ regeneration, as well as numerous pathological conditions. Angiogenesis undergoes multiple discrete steps that can be individually evaluated and quantified by a large number of bioassays. These independent assessments hold advantages but also have limitations. This article describes in vivo, ex vivo, and in vitro bioassays that are available for the evaluation of angiogenesis and highlights critical aspects that are relevant for their execution and proper interpretation. As such, this collaborative work is the first edition of consensus guidelines on angiogenesis bioassays to serve for current and future reference.
Original languageEnglish
Pages (from-to)425-532
Number of pages108
JournalAngiogenesis
Volume21
Issue number3
DOIs
Publication statusPublished - 1 Aug 2018

Keywords

  • Angiogenesis
  • Aortic ring
  • Endothelial cell migration
  • Proliferation
  • Microfluidic
  • Zebrafish
  • Chorioallantoic membrane (CAM)
  • Vascular network
  • Intussusceptive angiogenesis
  • Retinal vasculature
  • Corneal angiogenesis
  • Hindlimb ischemia
  • Myocardial angiogenesis
  • Recombinant proteins
  • Tip cells
  • Plug assay
  • Vessel co-option

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