Connective tissue growth factor and the cicatrization of cellular crescents in ANCA-associated glomerulonephritis

Marc Hilhorst, Helena M. Kok, Roel Broekhuizen, Pieter van Paassen, Peter van Breda Vriesman, Roel Goldschmeding, Tri Q. Nguyen, Jan Willem Cohen Tervaert*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

8 Citations (Web of Science)

Abstract

Background. Outcome in patients with anti-neutrophil cytoplasmic antibodies (ANCA)-associated glomerulonephritis (AGN) is difficult to predict. Scoring of renal biopsies has significant but limited predictive value. We investigated whether analysis of plasma and urine levels, and immunostaining of biopsies for the pro-fibrotic peptide connective tissue growth factor (CTGF), might improve prediction of renal outcome. Methods. ANCA-positive patients were included. Renal biopsies were classified according to the AGN classification. Biopsies were stained for CTGF protein. CTGF was measured by ELISA at the time of renal biopsy in plasma and urine, and during follow-up in plasma. Results. Eighty-two patients were included. CTGF staining was positive in crescentic lesions. Plasma CTGF at the time of renal biopsy was 2.4 +/- 1.7 pmol/mL when compared with 0.5 +/- 0.0 pmol/mL in healthy controls (P <0.01). Plasma CTGF was associated with cellular crescents, but not when corrected for renal function. Plasma CTGF at baseline was associated with fibrous crescents in the follow-up biopsy, also after correction for renal function. Plasma CTGF at baseline predicted renal survival more accurately than the AGN classification. Conclusion. In AGN patients, CTGF was overexpressed in crescentic glomeruli. Baseline plasma CTGF predicted the percentage of fibrous crescents in later biopsies, and renal survival, suggesting that CTGF is involved in the cicatrization, as opposed to resolution of cellular crescents in AGN.
Original languageEnglish
Pages (from-to)1291-1299
JournalNephrology Dialysis Transplantation
Volume30
Issue number8
DOIs
Publication statusPublished - Aug 2015

Keywords

  • ANCA
  • CTGF
  • glomerulonephritis
  • renal biopsy
  • vasculitis

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